J4 ›› 2009, Vol. 35 ›› Issue (6): 979-979-982.

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Effect of recombinant human basic fibroblast growth factor on angiogenesis of acute myocardial infarction in rats

 WANG Xiao-Jie1,2, LI Jing3, TIAN Hai-Shan1, MA Ji-Qing1, ZHOU Xin1, LIU Xiao-Ju1, WANG Yan-Fang1, TAO Na1, LI Jiao-Kun1,2   

  1. 1.ME Engineering Research Center of Bioreactor and Pharmaceutical Development,Jilin Agriculture University,Changchun |130118,China;2.Key Laboratory of |Radiobiology,Ministry of Health,School of Public Health,Jilin University,Changchun 130021,China;3.Department of Pharmacology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China)
  • Received:2009-06-10 Online:2009-11-28 Published:2009-11-28

Abstract:

Abstract:Objective
To investigate the effect of recombinant human basic fibroblast growth factor (rhbFGF) on myocardial angiogenesis in rats with acute myocardial infarction (AMI) and discuss the potential to extend treatment  to coronary artery disease patients who are not optimal candidates for conventional methods by therapeutic angiogenesis.Methods Myocardial infraction was induced in rats by using coronary artery ligation.A total of 60 rats were randomly divided into model group,rhbFGF-H group(50 mg•L-1),rhbFGF-L group (25 mg•L-1) and sham group;rhbFGF was directly injected into ischemic myocardium via some points.Morphometric and histological analysis were used to evaluate infarct size,vascular density and myocardial damage at 24 h,2 weeks and 4 weeks.Results The infarct sizes in the rhbFGF-H group and rhbFGF-L group were decreased significantly and lower than those in model group at 24 h,2 weeks and 4 weeks(P<0.05).The capillary densities in the infarct border zone in rhbFGF-H group and rhbFGF-L group were higher than those in model group and sham group at 2 weeks and 4 weeks(P<0.01,P<0.05).Hematoxylin-Eosine staining result showed that there were cell swelling,cardial musle fibers breakage,a part of myocyte cytolysis and inflammatory infiltration in the infarct border zone in model group at 24 h,2 weeks and 4 weeks
.Although there were cell swelling in rhbFGF-H group and rhbFGF-L group,but few cardial musle fibers breakage and myocyte cytolysis could be seen. Conclusion rhbFGF can promote the angiogenesis and decrease the infarct size in ischemic myocardium of rats with AMI.

Key words: myocardial infarction;recombinant human basic fibroblast growth

CLC Number: 

  • R542.22