Abstract:

Abstract:Objective
To study the function of AIRE on inducement and function of the CD4+CD25+Treg cells by regulating Notch1 expression,and discuss the mechanism of AIRE in peripheral immune tolerance.Methods  RAW264.7 cells were divided into transfected group and untransfected group,PEGFPC3-AIRE plasmid  was transfected with liposome,RT-PCR was used to detect the expressions of AIRE mRNA and Notch1 mRNA in every group.RAW264.7 cells were divided into transfected group,untransfected group,transfected and anti-Notch1 blocking group,untransfected and anti-Notch1 blocking group;FACS and RT-PCR were used to detect the number of CD4+CD25+Treg cells and the expression of Foxp3  mRNA in every group.Results AIRE gene was successfully transfected into the RAW264.7 cells.Notch1 mRNA expression in transfected group was significantly higher than that in untransfected group.The number of CD4+CD25+Treg cells and Foxp3 mRNA expression in transfected group were significantly higher than that in untransfected group,after applying the Notch1 antibody,compared with control group,the number of CD4+CD25+Treg cells and Foxp3 mRNA expression were lower in experimental group.
Conclusion AIRE could influence the production and function of CD4+CD25+Treg cells by up-regulation of Notch1 for keeping the function on peripheral immune tolerance.

Key words: autoimmune regulator；Notch1；CD4+CD25+Treg cells