Abstract:

Objective  To explore the antioxidant effect of honeysuckle    on hepatic RBL cells of rats in vivo  and in vitro and its underlying mechanism,and  provide theoretical basis for the application of honeysuckle in clinic.Methods The blood levels of T-AOC,SOD,GSH-Px,GSH or MDA in an antioxidant enzyme system were detected 1-2 h after administration in the established rat models,which were administrated with larger and smaller doses of honeysuckle respectively.The cultured human hepatic RBL cells were exposed to H2O2 as an oxidant and treated with 0.25g?L-1 neysuckle
before or after exposure to H2O2.The cell survival and apoptosis were detected by MTT and TUNEL assays.The expression levels of NF-kB,Bcl-2,Bax,HSP-70 and  Caspase-3 were determined by immunohistochmistry and Western blotting and the cell anti-oxidant enzyme system was detected by corresponding kits.Results In the antioxidant and anti-apoptotic effects of honeysuckle,the pre-protective effect was markedly higher than the post-protective.The expressions of NF-κB and HSP-70,the expression ratio of Bcl-2/Bax were down-regulated,and the apoptotic rate in  pr
e-protective group was lower than that in  post-protective group.  Besides,the detected cell level of anti-oxidant enzyme system was up-regulated significantly.In the plasma of rat model the levels of T-AOC,GSH-Px,GSH and SOD were promoted(P<0.05),while that of MDA was decreased markedly(P<0.05).Conclusion  Honeysuckle displays antioxidant capacity through up-regulating the antioxidant enzyme system and down-regulating NF-κB signal transduction pathway.

Key words: honeysuckle；hydrogen peroxide；antioxidant enzyme system； anti-apoptoic signal transduction；human hepatic RBL cell line