Abstract:

Objective
To study the effects and mechanism of Yunnan Baiyao on the inflammatory response and satellite cell regeneration after acute injury of skeletal muscle in rats. Methods
Rat models of skeletal muscle injury (gastrocnemius, fast twich;soleus,slow twich) induced by treadmill were constructed 24 h after establishment of the models,  the left sides of hind limbs in rats were treated with   Yunnan Baiyao, and the right sides were treated with saline at the equal volume.4 rats were chosen 2,4,7,10,14 d after injury,respectively,and  gastrocnemius and soleus of the treated sides (the left sides) and untreated sides (the right sides) from hind limbs of 4 rats were obtained,and stained with  immunohistochemistry and HE. The quantity   and morphological changes  of inflammatory cells and satellite cells  were observed under optical microscope. Results Compared with untreated sides, the number of inflammatory cells of fast twich after injury in  the treated sides was  decreased obviously at the  2nd,4th,7th day after injury(P<0.01) and the number of inflammatory cells of slow twich  at the 2nd day  after injury in treated sides was  decreased (P<0.01);the  necrotic cells of skeletal muscle were decreased.Compared with untreated sides  the muscle  fibers of fast twich  and slow twich  were repaired at the 7th,10th day,and the number of satellits cells was increased obviously in the treated sides(P<0.01). Conclusion Yunnan Baiyao can inhibit the destruction of inflammatory cells to injuried  muscle tissues, shorten the inflammatory reaction process and promote the proliferation of satellite cells and muscle fiber repairment after injury.

Key words:  Yunnan Baiyao, acute injury of skeletal muscle；inflammatory cells, satellite cells