Abstract:

Objective
To study the effect of Tongbiding freezing-dryied  powder  for injection on the function of WDR neuron of spinal cord in living rats with chronic constriction injury (CCI), and  clarify its analgesia mechanism in  spinal cord  center, and further explore   whether it has the drug dependence at  the cellular level. Methods Electricity physiology extracellular recording technique was used , in the CCI living  rat’[KG-*3]s expanded spinal cord waist stage  the electricity activity of identical WDR neuron cells was recorded  before and after administration of 20 mg.kg-1 Tongbiding. The electric discharge number of C response was observed before  and  2, 4, 8, 10 min after administration. The spontaneous electric dischare number. was observed  before and   1, 2, 4, 6 min after administration; wind-up phenomenon was also observed.Results The electric discharge number of C response  was obtained
 in  8 WDR neurons,three were significant differences of the mean electric discharge number of C response  between 2,4,8 min after administration and before administration (P<0.05 or P<0.01). The observation of cell’[KG-*3]s spontaneous ele
ctric discharge number recorded  12 cells altogether, 6 cells stopped discharging spontaneously immediately after administration, 6 cells stopped discharging spontaneously  6 min after administration. There were significant differences of the mean  spontaneous electric discharge number  between 2, 4 min after administration and before administration  (P<0.01).Five  wind-up phenomena were found altogether, this phenomenon was suppressed after .
 Conclusion The analgesia effect of Tongbiding freezing-dryied   powder  for injection on spinal cord  center  is related to affecting the WDR neuron, and it is confirmed  that the drug does not have drug dependence  at  the cellular level.

Key words: Tongbiding freezing and drying medicine powder；CCI model rat；WDR neuron, electricity activity