Abstract:

Abstract:Objective
To study the influence of aprotinin in hepatocyte proliferation ability in rats with experimental chronic liver injury and provide thoreotical basis for study on protective effect of aprotinin on chronic liver injury.  Methods Wistar rats were randomly divided into normal control group,carbon tetrachloride (CCl4) model group,low dose  aprotinin group,midde dose aprotinin group,high dose aprotinin group and hepatocyte group.Immunohistochemical method was used to observe the proliferating cell nuclear antigen (PCNA)-positive cell number in various groups.  Results The positive cell number in control group was (4±2)/vision;(5±2)/vision in model group;(39±13)/vision,(49±14)/vision and (57±12)/vision in low,middle,and high dose groups;(26±8)/vision in hepatocyte group. The positive cell numbers in aprotinin groups were markedly increased compared with model group（P<0.05）,the positive cell number was increased with the increase of aprotinin dose. The positive cell numbers in aprotinin groups were more than that in hepatocyte group（P<0.05）.The proliferation of PCNA positive cells in each aprotinin group was significantly increased,especially in high dose aprotinin group. Conclusion The aprotinin could promote the proliferation of chronicly injured liver cells and this effect could be enhanced by the increasing of aprotinin dose.

Key words: aprotinin;carbon tetrachloride;chronic hepatic injury;rats,Wistar;immunohistochemistry;proliferation