Abstract:

Abstract:Objective
To evaluate the effects and mechanisms of taurolidine in combination with X-rays irradiation on cell cycle progress of murine melanoma cells,and  provide a new idea of malignant tumor clinical treatment. Methods B16-4A and B16-F10 cells were divided into 4 groups according to the  concentrations of taurolidine (0,25,50 and 100 μmol.L^-1 ),respectively and combined with 1,2 and 4 Gy X-rays irradiation. The apoptotic  rate and cell cycles were detected by flow cytometry. The expressions of cyclinB,cdc2,and caspase-3 were detected by Western blotting. Results  Compared with 25  μmol.L^-1  taurolidine  group,the arrest of G2/M phase of B16-4A and B16-F10 cells after treated with 50 and 100  μmol.L^-1 taurolidine was found and the cell numbers  were increased by 54.9%,73.7% and 36.8%，55.5%,respectively(P<0.05).The arrest of G2/M phase of B16-4A and B16-F10 cells was also found and the cell numbers were decreased by 52.1%，44.2% and 59.3%，52.7%(P<0.05),respectively, after treated with  50  μmol.L^-1  taurolidine in  combination with 2 and 4 Gy X-rays irradiation. Therefore,the increase of caspase 3 expression  and the decrease of cyclin B and cdc2 expressions were induced by the combination of 50  μmol.L^-1  taurolidine with 2 and 4 Gy X-rays irradiation  compared with control,taurolidine alone and irradiation alone groups.Conclusion Taurolidine in combination with X-rays irradiation can delete the _G2/M arrest,supress the expressions of cyclin B and cdc2,increase the expression of caspase-3 and induce the apoptosis.

Key words: taurolidine;X-rays;cell cycle;cell division cycle 2；cyclin B