Abstract:

Abstract:Objective
To study the effects of 20(s)-proto-panaxdiol（PPD）on transcription and protein expressions of caspase-9 and caspase-3,and content of cleaved caspase-3 in Siha cells in vitro,and clarify the mechanism of its apootosis action. Methods Siha cells cultivated in vitro were treated with alcohol (negative control group)and 20 μg?L-1 PPD (20  μg.L^-1 PPD group),respectively. The apoptosis was detected by FCM 48 h after treatment. The transcription and protein expressions of caspase-9 and caspase-3 in siha cells were analyzed by RT-PCR,Western blotting and immunocytochemical staining. Results Compared with negative control group,after treated with 20 μg?L-1 PPD for 48 h,the apoptotic rate of Siha cells was increased (P<0.05),the transcription and protein expressions of caspase-9 and caspase-3 were up-regulated(P<0.01),and the content of cleaved caspase-3 was increased(P<0.01). Cell immunochemistry detection showed the  brown particles in the experimental  cells in 20  μg?L-1 PPD group. Conclusion PPD may induce the apoptosis of Siha cells,and the mechanism may be related to the up-regulation and activition of caspase family.

Key words: 20(s)-proto-panaxdiol;Siha cells;apoptosis；caspase-3;caspase-9;gene expression