Journal of Jilin University Medicine Edition

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Detection of 16S rRNA methylase genes in gram-negative bacilli isolated from hospitals in Changchun area and its significance

ZHOU Jia-qi1,SHI Hong-yan1,XU Hua1,HUANG Jin-feng2,SUN Yan-bo1   

  1. 1.Department of Pathogenobiology,Norman Bethune College of Medicine,Jilin University,Changchun 130021,China;2.Corporation of KeXing Vaccine Technology,Dalian 116000,China
  • Received:2013-04-12 Online:2013-09-28 Published:2013-12-13

Abstract:

Abstract:Objective To analyze the distribution and genotypes of 16S rRNA methylase genes in gram-negative bacilli isolated from hospitals in Changchun area and to provide a reasonable basis for the application of aminoglycosides. Methods  Amikacin-resistant and gentamicin-resistant strains were screened using agar dilution tests,and 16S rRNA methylase genes in gram-negative bacilli isolates were amplified by PCR and genotypes of 16S rRNA methylases (armA,rmtB,rmtA,rmtC,rmtD and npmA) were analyzed using gene sequencing.Results The resistant rates of 116 clinical isolates of gram-negative bacilli to amikacin and gentamicin were 42.2% and 75.0%,respectively. Fifty of 116 isolates were positive for 16S rRNA methylase genes(43.1%,50/116). The common genotypes of 16S rRNA methylase were armA and rmtB (12.1% and 31.0%);however,the rmtA,rmtC,rmtD and npmA genes were not found.Conclusion The genotypes of 16S rRNA methylase in gram-negative bacilli in Changchun area are armA and rmtB which  is one of the reasons to induce high resistance to aminoglycosides.

Key words: gram-negative bacilli, 16S rRNA methylase, antibiotic-resistance

CLC Number: 

  • R378