Abstract:

Objective To observe the effect of  gypenosides-containing serum on the expressions of  p38 mitogen-activated protein kinase（ p38MAPK）protein and c-Jun mRNA in photoaging HaCaT cells and to explore the mechanism of  inhibitory effect of gypenosides-containing serum on photoaging.Methods  The photoaging HaCaT cell model was established by UVB radiation, low,middle,and high  doses    of gypenosides-containing  serum were used in the culture fluid of phatoaging HaCaT cell models;meanwhile blank control group,blank serum group and UVB model group were set up.The expression levels of p38MAPK protein and c-Jun mRNA of cells in each group were measured by Western blotting and RT-PCR methods. Results  Compared with blank control group,the expression levels of p38MAPK protein and c-Jun mRNA of the cells  inUVB model group  were significantly increased,the difference had statistical significance（P<0.01）.Compared with UVB model group,the expression levels of p38MAPK protein and c-Jun mRNA of cells in low,middle and high doses of   gypenosides-containing serum groups were significantly decreased,the difference had statistical significance（P<0.01）.Compared with blank serum group,the expression levels of p38 MAPK protein and c-Jun mRNA of the cells in low,middle and high doses of gypensides-containing serum groups were decreased(P<0.01).Conclusion Gypenosides-containing serum could prevent the skin photoaging by inhibinting the expressions of p38MAPK protein and c-Jun mRNA.

Key words: photoaing, gypenosides;HaCaT, ultraviolet B;p38MAPK, c-Jun