Abstract:

Abstract:Objective To study the expression of acidic fibroblast growth factor(FGF-1) in MCF-7 cells mediated by lentivirus system,and obtain a primarily stable cell line highly expressing FGF-1 and  to lay foundation for further exploring the role of FGF-1 in the tumorigenesis and development of breast cancer. Methods The lentivirus-expressing plasmid pWPXLd-FGF-1 was constructed and cotransfected into 293FT cells with lentiviral vector psPAX2,pMD2.G and pWPXLd-FGF-1. The lentivirus were collected and infected MCF-7 cells,the expression of FGF-1 was tested by flow cytometry and Western blotting. The capabilities of proliferation and migration of MCF-7 cells transfected with FGF-1 were also examined by wound healing test.  Results The pWPXLd-FGF-1 plasmid was successfully constructed. The lentivirus was obtained and the titer of lentivirus reached to 2.8×10⁶ T U•mL^-1. In addition,a cell line  highly expressing FGF-1 was obtained,its transfection efficiency was more than 60%,which promoted the  proliferation and migration of MCF-7 cells.  Conclusion A lentivirus vector of FGF-1 is sucessfully constructed,and it can higly  express in MCF-7 cells. 

Key words: acidic fibroblast growth factor;lentivirus;breast neoplasms;wound healing