Abstract:

To observe the protective effect of Panaxadiol Saponins (PDS) on rabbit heart failure model induced by acute alcohol infusion, and to explore its action mechanism of protecting myocardium.Methods 15 healthy rabbits were randomly divided into control group,model group and PDS group,5 rabbits in each group.The rabbits in control group were given 0.2 g•mL^-1 saline by intravenous drip at constant speed,the rabbits in model group were given 20% ethanol with same method,and the rabbits in PDS group were given 0.025 g•kg^-1　PDS by intravenous injection before intravenous drip of 20% ethanol.The  hemodynamic changes were observed by ventricular intubation； the levels of serum lactate dehydrogenase (LDH),creatine kinase (CK),and creatine kinase isoenzyme (CKMB) were determined by colormetric method.The level of  malondialdehyde (MDA) in myocardial tissue  homogenate,the activities of superoxide dismutase (T-SOD),glutathione peroxidase (GSH-Px) and catalase (CAT)  were also detected. Results Compared with control group,the left ventricular end-diastolic pressure (LVEDP) of the rabbits in model group was significantly decreased at 30 min(P<0.05);the serum LDH,CK and CKMB levels were increased(P<0.05),the MDA level in myocardial tissue homogenate was increased(P<0.05),and the T-SOD,GSH-Px and CAT activities were decreased (P<0.05).Compared with model group,the LVEDP of the rabbits in PDS group was increased,the serum LDH,CK,and CKMB levels were decreased(P<0.05),the MDA level was decreased(P<0.05),and the activities of T-SOD,GSH-Px and CAT were increased(P<0.05).Conclusion PDS has protective effect on heart failure induced by acute alcohol infusion,and its mechanism may be related to the improvement of cardiac peroxidation.

Key words: panaxadiol saponins, alcohol, myocardial injury, heart failure, lipid peroxidation