营养素复合物对酒精性肝病大鼠的保护作用及其机制

doi:10.13481/j.1671-587x.20190611

Abstract

Abstract: Objective: To explore the protective effect of nutrient complex on the rats with alcoholic liver disease(ALD),and to elucidate its mechanism. Methods: A total of 60 male Wistar rats were randomly divided into blank control group,model control group,low,medium and high doses (2.5,5.0,and 15.0 mL·kg^-1)of nutrient complex groups,and there were twelve rats in each group. The rats in blank control and model control groups were fed with distilled water and the rats in other groups were fed with different doses (2.5,5.0,and 15.0 mL·kg^-1)of nutrient complexes. The rats in blank control group were fed with distilled water again and the rats in other groups were fed with 30% wine 2 h later. The rats in various groups were administrated by gavage twice a day for 36 d.The liver tissue of the rats in various groups were taken and the levels of malonaldehyde(MDA),reduced glutathione(GSH) and triglyceride(TG) in liver tissue of the rats in various groups were detected.The pathological morphology of liver tissue of the rats in various groups was observed, Sudan Ⅲ staining was used to observe the number of lipid droplets in liver tissue and liver cells and the distribution of hepatic fat of the rats in various groups,and the steatosis of liver tissue of the rats in various groups was scored. Results: Compared with blank control group,the levels of MDA and TG in liver tissue of the rats in model control group were significantly increased(P<0.01),and the level of GSH was significantly decreased(P<0.05); compared with model control group,the levels of MDA and TG in liver tissue of the rats in low,medium and high doses of nutrient complex groups were decreased significantly(P<0.01),and the levels of GSH in liver tissue of the rats in medium and high doses of nutrient complex groups were significantly increased(P<0.05 or P<0.01). Large amounts of lipid droplets were found in the liver tissue and liver cells of the rats in model control group,the nucleus was blue and presented the pathological changes of steatosis of liver tissue. Compared with blank control group,the number of lipid droplets in liver tissue and liver cells of the rats in model control group was increased,and the pathological score of steatosis of liver tissue was increased(P<0.05); compared with model control group,the number of lipid droplets in liver tissue and liver cells of the rats in medium and high doses of nutrient complex groups was significantly decreased,and the pathological scores of steatosis of liver tissue were decreased (P<0.05). Conclusion: Nutrient complexes play a protective role in ALD by increasing the levels of antioxidants and reducing the peroxidation products in the liver tissue.

Key words: nutrient complex, alcoholic liver disease, malonaldehyde, reducing glutathione, triglyceride

CLC Number:

R155.3

CHAI Bowen, YANG Yuefeng, YANG Yetong, ZHOU Yang, ZHANG Yezhuo, LIU Huafeng, LIU Yajuan. Protective effect of nutrient complex on alcoholic liver disease rats and its mechanism[J].Journal of Jilin University(Medicine Edition), 2019, 45(06): 1256-1260.

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Protective effect of nutrient complex on alcoholic liver disease rats and its mechanism

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