Journal of Jilin University Medicine Edition ›› 2016, Vol. 42 ›› Issue (04): 671-675.doi: 10.13481/j.1671-587x.20160407

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Effect of Angelica Sinensis Volatile Oil on miRNA differential expression profiling in myocardium tissue in spontaneously hypertensive rats

YI Lin1, CHEN Beibei1, SUN Shaobo2, YANG Long3, JI Lufeng1, SHI Xianghui1   

  1. 1. Department of Internal Medicine, College of Integrated Traditional and Western Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, China;
    2. Department of Medical Biology, College of Basic Medical Sciences, Gansu University of Chinese Medicine, Lanzhou 730000, China;
    3. Department of Surgery, Tongwei People's Hospital, Gamsu Province, Tongwei 743300, China
  • Received:2015-11-20 Published:2016-07-20

Abstract:

Objective: To explore the effect of Angelica Sinensis Volatile Oil on the mi RNA expression profiling in myocardium tissue of the spontaneously hypertensive rats (SHRs), and to clarify the possible mechanism of Angelica Sinensis Volatile Oil. Methods: All the SHRs were divided into Angelica group,model group and captopril group,and other Wistar rats with the same age were selected as control group. The non-invasive systolic blood pressure was detected; after 4 weeks of administration,the changes of miRNA expression profiling in myocardium tissue were measured by Affymetrix miRNA 4.0 Array. KEGG analysis was used to identify the target genes. Results: Compared with control group,the systolic blood pressure of the rats in model group was significantly decreased (P < 0.05) before treatment. 4 weeks after administration,compared with model group,the systolic blood pressure of the rats in Angelica group was significantly decreased(P < 0.05). Compared with model group,29 differential miRNAs of rats in Angelica group were found(P < 0.05),with 13 up-regulated miRNAs and 16 down-regulated miRNAs. The KEGG analysis results showed that miR-19a,let-7i, and miR-181c were related to insulin signaling pathways;let-7i,miR-181a,and miR-455 were related to VEGF signaling pathways; miR-122,miR-181a,miR-200b,miR-181c,let-7i,and miR-19a were related to apoptosis(P < 0.05). Conclusion: Angelica Sinensis Volatile Oil can decrease the blood pressure in SHRs and it can regulate the blood pressure by regulating the miRNA related to insulin signaling pathways and VEGF signaling pathways.

Key words: Angelica Sinensis Volatile Oil, rat,inbred SHR, miRNA expression profiling

CLC Number: 

  • R544.1