Abstract:

Objective: To detect the association between the single nucleotide polymorphisms(SNPs)of PLA2G6 gene of Ca²⁺-independent phospholipase A2(iPLA2)gene family and the incidence of type 2 diabetes mellitus(T2DM)in Chinese northern Han people,and to discuss whether the PLA2G6 gene was the susceptible gene of T2DM.Methods: 169 patients with T2DM were selected as case group and 199 healthy controls without T2DM were selected as control group.The techniques of polymerase chain reaction (PCR)and restriction fragment length polymorphism (RFLP)were used to examine the genotypic and allic frenquencies of rs2235346,rs2272831,and rs2284060 sites at PLA2G6 gene of the objects in case and control groups.Results: The distribution of genotypic and allic frequencies of rs2235346,rs2272831,and rs2284060 sites at PLA2G6 gene did not deviate from Hardy-Weinberg equilibrium in both case and control groups (P>0.05).The distribution of genotypic and allic frequencies of rs2235346,rs2272831,and rs2284060 sites at PLA2G6 gene had no significant differences between case and control groups(χ²=0.604,P>0.05).Conclusion: There is no significant relationship between the genotypic and allic frequencies of rs2235346,rs2272831,rs2284060 sites at PLA2G6 gene and T2DM,and the PLA2G6 gene is not the susceptible gene of T2DM.

Key words: diabetes mellitus,type 2, phospholipase A₂, susceptible gene