Journal of Jilin University Medicine Edition ›› 2016, Vol. 42 ›› Issue (02): 245-249.doi: 10.13481/j.1671-587x.20160211

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Potential to differentiation of human dermis-derived fibroblast-like cells into mesenchymal stem cells in vitro

LYU Shuang1, XU Jinying1, FENG Wei2, ZHANG Zechuan1, LI Meiying1, BI Bo3, SUN Dongjie1, LI Yulin1, CHI Guangfan1   

  1. 1. Department of Pathology, School of Basic Medical Sciences, Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun 130021, China;
    2. Department of Orthopedics, First Hospital, Jilin University, Changchun 130021, China;
    3. Department of Biotissue Engineering, School of Pharmacy, Jilin University, Changchun 130021, China
  • Received:2015-09-10 Published:2016-03-31

Abstract:

Objective: To study the differentiation capacity of the fibroblast-like cells isolated from human skin dermis into mesenchymal stem cells, and to explore the feasibility to use these cells as alternative cell source of autologus bone marrow mesenchymal stem cells (BMSCs) for regeneration of tissue injuries and defects.Methods: Full thickness skin samples were obtained from the abdomen of surgical patients, then digested with dispase and collagenase Ⅰ subsequently.Thereafter, the digested cells were collected and cultured, followed by suspension with serum free medium containing N2, B27, basic fibroblast growth factor(bFGF), and epidermal growth factor(EGF).The skin dermis derived spheroids(SDDSs) were collected and monolayer cultured in serum-containing medium.Finally, the cells were characterized by immunofluorescence staining and differentiation assays.Results: The dermis derived cells proliferated and formed SDDSs in the suspension of serum-free medium.After monolayer cultivation in serum-containing medium, the cells from spheroids were successfully expanded to large number.The cells expressed mesenchymal stem cells markers CD90, CD105 and vimentin.Under osteogenic, chondrogenic and adipogenic differentiation conditions, these cells were differentiated into the alizarin red, safranin O, and oil red O staining positive cells, displayed similar differentiation traits with BMSCs.However, safranin O staining was weaker in the dermis derived cells than BMSCs.Conclusion: A kind of fibroblast-like cells exist in human skin dermis, and have osteocytic, chondrogenic and adipogenic differentiation potentials, demonstrating that these cells will be utilized as a novel cell source for repairing the tissue injury and defect in clinic.

Key words: skin, dermis, mesenchymal cells, chondrocytes, bone cells

CLC Number: 

  • R318