冠心病伴心外膜脂肪增厚大鼠模型的制备

doi:10.13481/j.1671-587x.20170135

Abstract

Abstract:

Objective: To build the SD rat models of coronary atherosclerotic heart disease (CAD) complicated with epicardial adipose tissue (EAT) thickening, and to lay the foundation for the study on the role of EAT in CAD.Methods: Fifty healthy male SD rats were randomly divided into control group(n=15) and model group (n=35). The rats in model group were fed with high fat diet; the rats were given i.p. injection of vitamin D3 in a dose of 200 000 IU·kg^-1 monthly for consecutive 3 months, a total of 4 times. The rats in control group were administrated with the same volume of normal saline through the same route and were fed with normal diet. It took 210 d to build the models. The general condition, body weights, blood lipid levels, epicardial fat thickness and pathological changes of myocar clium tissue were detected.Results: The general condition showed that the hair of the rats in model group was shading and the activities were decreased.At 90-180 d, the body weights were slowly increased,and at 180-210 d, the body weights were gradually decreased. Compared with control group, the EAT thickness, the serum low-density lipoprotein cholesterol (LDL-c), total cholesterol (TC), triglyceride (TG) levels and atherosclerosis index(AI) of the rats in model group were significantly increased(P<0.05 or P<0.01), while the high-density lipoprotein cholesterol (HDL-c) level was significantly decreased (P<0.05 or P<0.01). No intima-media thickness and vascular stenosis of the coronary artery were found in control group and the vessel wall was complete. The atherosclerotic plaque and the intima thickness were not found in myocardial small vessel as well. However, the endothelial damage, inflammatory cell infiltration and lipid deposition, atherosclerotic plaques and calcification in the coronary artery wall of the rats in model group were found;the intima-media thickness was increased, coronary stenosis was above 85%, and a large number of foam cells were found in the intima which was thickened and damaged. Conclusion: The stable and reproducible SD rat models of CAD complicated with EAT thickening are successfully established, which can be used to study the role of EAT in the pathophysiology and molecular biology of CAD.

Key words: epicardial adipose tissue, coronary atherosclerotic heart disease, disease model, animal

CLC Number:

R-332

LIU Yang, HUAI Shitao, FU Wenbo, SONG Yaqin, LU Mu, PANG Lijuan, WEI Yutao. Establishment of rat models of coronary atherosclerotic heart disease complicated with epicardial adipose tissue thickening[J].Journal of Jilin University Medicine Edition, 2017, 43(01): 176-180.

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Establishment of rat models of coronary atherosclerotic heart disease complicated with epicardial adipose tissue thickening

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