miR-139-5p在小细胞肺癌组织中的表达及其临床意义

doi:10.13481/j.1671-587x.20160520

Abstract

Abstract:

Objective: To explore the expression of miR-139-5p in small cell lung cancer (SCLC)tissue and its clinical significance,and to clarify the role of miR-139-5p in the occurrence and development of SCLC. Methods: The biological function of miR-139-5p was examined by cell growth, apoptosis and cell cycle analysis.The expressions of miR-139-5p in 50 cases of cancer tissue and paracarcinoma normal tissue were examined by QRT-PCR. Combined with the clinical data,the role of miR-139-5p in clinic was anzlyzed. Results: The expression level of miR-139-5p in SCLC tumor tissue was lower than that in normal lung tissue (P<0.01). The expression level of miR-139-5p in the cells was un-regulated significantly after transferred miR-139-5p mimics(P<0.01).Compared with control group,the proliferation abilitiy of cells was reduced after up-regulating the expression of miR-139-5p (P<0.01),the number of cells at G₁ phase was increased(P<0.05),and the cells were arrested at G₀/G₁ phase. The miR-139-5p expression was not associated with gender and age(P>0.05). The expression level of miR-139-5p in the patient at LD stage was lower than that of the patients at ED stage (P<0.01).The expression level of miR-139-5p in the resistant patients was higher than that of the patients sensitive to chemotherappy(P<0.01).The expression level of miR-139-5p in the survival patients was lower than that in the death patients(P<0.01). Cox regression analysis indicated that miR-139-5p expression and disease stage were the independent prognostic factors for SCLC. Conclusion: miR-139-5p in participates in the occurrence and development of SCLC by inhibiting the cell proliferation, promoting apoptosis and inducing the cell cycle arrest;it may be used as a target gene to evaluate the prognosis of SCLC patients.

Key words: cancer,small cell lung, real-time quantitative polymerase chain reaction, miR-139-5p

CLC Number:

R734.2

LIU Huanxin, ZHANG Guoxiang, GUO Linlang, TAN Songshan. Expression of miR-139-5p in small cell lung cancer tissue and its clinical significance[J].Journal of Jilin University Medicine Edition, 2016, 42(05): 942-948.

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Expression of miR-139-5p in small cell lung cancer tissue and its clinical significance

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