Abstract:

Abstract:Objective
To study the expression of Brg1 in laryngeal squamous cell carcinoma (LSCC) tissue and explore its relationship with the occurrence and development of laryngeal cancer.  Methods Immunohistochemical method was used to detect the expressions of Brg1 in 30 cases of LSCC  and 10 cases of paracarcinoma normal mucosa. Results  The expression of Brg1 in paracarcinoma normal mucosa  was positive(the grey value was  124.60±4.51),and the expression of Brg1 in LSCC was low(the grey value was 159.20±7.69),the difference between  two groups was significant（P<0.01）. The　grey values expressed by Brg1 in well,moderately and poorly differentiated LSCC were 132.80±1.92,165.20±2.59,and 179.60±1.52,respectively;there were significant differences betwen moderately,poorly differentiated LSCC and  well differentiated LSCC (P<0.01). The grey values expressed by Brg1 in stage Ⅰ-Ⅱ,stage Ⅲ  and stage  Ⅳ  LSCC were 145.30±2.36,165.20±4.31,and 179.4±3.03,respectively;there were significant differences between stage Ⅲ,stage Ⅳ and  stage  Ⅰ-Ⅱ （P＜0.05）.The grey values expressed by Brg1 in vascular invasion group and no vascular invasion group were 176.80±3.48 and 143.50±8.26,the difference between  two groups was significant（P<0.01）. The grey values expressed by Brg1 in lymph node metastasis group and no lymph node metastasis group were 155.60±4.02 and  161.70±3.52,there was no significant difference between  two groups(P>0.05).   Conclusion  The expression of Brg1 in LSCC is low,and it is related to the development of cancer and pathological factors,which may be an important factor in laryngeal cancer.

Key words: laryngeal neoplasms;Brg1;immunohistochemistry