Abstract:

Objective
To detect the changes of FOXD1 expression in invasive breast cancer tissues and tissues surrounding breast cancer,and provide basis for further study on the functions of FOXD1 in the occurrence and development of breast cancer. Methods 113 samples were classified into invasive breast cancer tissue and tissues surrounding breast cancer by frozen section and HE staining.Then the expression levels in four samples of invasive breast cancer tissues and another four samples of tissues surrounding breast cancer were examined by qRT-PCR and Western blotting respectively.Results 113 samples were classified into 36 cases of invasive breast cancer tissues and 77 cases of tissues surrounding breast cancer according to standards of pathohistology.The qRT-PCR result showed that the FOXD1 expression in invasive breast cancer tissues was increased significantly compared with tissues surrounding breast cancer (P<0.01).The result of Western blotting also demonstrated that the FOXD1 expression in invasive breast cancer tissues was higher than that in tissues surrounding breast cancer (P<0.05).Conclusion The expression of FOXD1 in breast cancer tissue is significantly higher than that in  tissues surrounding breast cancer. The result indicates that the expression level of FOXD1 is related to the occurrence and development of breast cancer.

Key words: FOXD1;breast neoplasms;Western blotting;qRT-PCR