The data were downloaded from the GEO database and the GSE126094 chip data contained 10 samples of colorectal cancer tissues and 10 samples of adjacent cancer tissues. R software was used to select the differentially expressed circRNAs； the miRNAs that bound to differentially expressed circRNAs were found in the CSCD database；Perl software was used to predict the target genes of the miRNAs； GO biological function enrichment analysis and KEGG pathway enrichment analysis were performed in the target genes.

Compared with adjacent cancer tissue samples，there were 23 circRNAs with increasing expression levels （logFC>2 and P<0.05） and 36 circRNAs with decreasing expression levels （logFC<－2 and P<0.05） in the colorectal cancer tissue samples. The expression level of hsa_circ_0043278 was decreased significantly（logFC=－7.481 and P<0.05）. There were 66 miRNAs combined with hsa_circ_0043278，and the target genes were predicted. The GO enrichment analysis results showed that the target genes were mainly involved in Wnt-mediated cell signal transduction；the KEGG pathway enrichment analysis results showed that the target genes mainly enriched in PI3K-AKT signaling pathway.

The expression level of hsa_circ_0043278 in the colorectal cancer tissue samples is significantly decreased， which indirectly affect the functions of target genes such as UTP18， CLIP4 and STC2， and further affect the regulation of Wnt-mediated cell signal transduction and PI3K-AKT signaling pathway， and finally reduce the inhibitory effect on the colorectal cancer.