人结直肠癌和肿瘤引流淋巴结组织中Foxp3+调节性T细胞和髓样树突状细胞的表达及其意义

doi:10.13481/j.1671-587x.20190325

Abstract

Abstract: Objective:To explore the expressions of Foxp3⁺ Tregs and CD11c⁺ mDCs in the colorectal cancer (CRC) tissue and their infiltrations in the tumor draining lymph node (TDLN) tissue,and to explore the clinical significances. Methods:Fifty-two samples of surgical resection of the CRC patients were collected. The expressions of Foxp3⁺ Tregs and CD11c⁺ mDCs in the specimens of the CRC patients and TDLN tissue were detected by immunohistochemistry, and the relationship between the expression frequencies of Foxp3⁺ Tregs and CD11c⁺ mDCs and the clinicopathological characteristics of the patients were discussed. Results:The positive expression frequency of Foxp3⁺ Tregs in cancer tissue of the CRC patients was higher than that in adjacent normal mucosa tissue(P<0.01); The expression frequency of Foxp3⁺ Tregs in cancer tissue in the patients with positive lymph node metastasis was higher than that in the patients with negative lymph node metastasis(P<0.01); the expression frequency of Foxp3⁺ Tregs in the patients in TNM stage Ⅲ+Ⅳ was higher than that in TNM stage Ⅰ+Ⅱ(P<0.01); the positive expression frequency of Foxp3⁺ Tregs in metastatic TDLN (mTDLN) tissue was higher than that in metastatic free TDLN (mfTDLN) tissue(P<0.01). The positive expression frequency of CD11c⁺ mDCs in cancer tissue of the CRC patients was higher than that in adjacent normal mucosa tissue(P<0.01); the positive expression frequency of CD11c⁺ mDCs in the patients with positive lymph node metastasis was lower than that in the patients with negative lymph node metastasis(P<0.01);the positive expression frequency of CD11c⁺ mDCs in the patients in TNM stage Ⅲ+Ⅳ was lower than that in the patients in TNM stage Ⅰ+Ⅱ(P<0.01); the positive expression frequencies of CD11c⁺ mDCs in cancer tissue with different depths invasion were significantly different (P<0.05);the positive expression frequency of CD11c⁺ mDCs in mTDLN tissue was lower than that in mfTDLN tissue (P<0.01).There were no significant correlations between the expressions of Foxp3⁺ Tregs and CD11c⁺ mDCs in the CRC and TDLN tissues(P>0.05). Conclusion:The occurrence and development of CRC are related to the changes of Foxp3⁺ Tregs and CD11c⁺ mDCs expression frequencies in the local microenvironment of cancer tissue.Foxp3⁺ Tregs and CD11c⁺ mDCs play a role in inhibiting the cellular immunity in tumor microenvironment.

Key words: colorectal neoplasms, tumor draining lymph nodes, tumor metastasis, regulatory T cells, myeloid dentritic cells

CLC Number:

R735.35

WANG Dan, SONG Ziqi, LI Yifei, LI Chun, DONG Zhiheng, DONG Ying, GAI Xiaodong. Expressions of Foxp3⁺ regulatory T cells and myeloid dentritic cells in human colorectal cancer and tumor draining lymph node tissues and their significances[J].Journal of Jilin University(Medicine Edition), 2019, 45(03): 621-626.

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Expressions of Foxp3⁺ regulatory T cells and myeloid dentritic cells in human colorectal cancer and tumor draining lymph node tissues and their significances

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Expressions of Foxp3+ regulatory T cells and myeloid dentritic cells in human colorectal cancer and tumor draining lymph node tissues and their significances

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Expressions of Foxp3⁺ regulatory T cells and myeloid dentritic cells in human colorectal cancer and tumor draining lymph node tissues and their significances