Journal of Jilin University(Medicine Edition) ›› 2021, Vol. 47 ›› Issue (4): 934-942.doi: 10.13481/j.1671-587X.20210416
• Research in basic medicine • Previous Articles Next Articles
Tengfei LI,Shiyu WANG,Xinru JIANG,Miao SUN,Tingting LI,Jiafeng LIN,Ying WANG,Yonggang LI,Xiaoli TAO()
Received:
2020-10-19
Online:
2021-07-28
Published:
2021-07-22
Contact:
Xiaoli TAO
E-mail:taoxiaoli@jzmu.edu.cn
CLC Number:
Tengfei LI,Shiyu WANG,Xinru JIANG,Miao SUN,Tingting LI,Jiafeng LIN,Ying WANG,Yonggang LI,Xiaoli TAO. Analysis on expression characteristics of non-structural protein μNS and σNS of Nelson Bay orthoreovirus[J].Journal of Jilin University(Medicine Edition), 2021, 47(4): 934-942.
Tab.1
Primer squences of each target gene in PCR detection"
Primer | Sequence |
---|---|
pEFHAσNS | F 5'-CGCCGAATTCGGATCaAATGCGTGTCGGTGTCTCGAGAAA-3' R 5'-CGACATCGATGGATCTTATGCGATGCGGCCTGGTCTGACA-3' |
pEFHAσNS△N10 | F 5'-CGCCGAATTCGGATCaAGGTACTGGGTCTCAGACCATACTAC-3' R 5'-CGACATCGATGGATCTTATGCGATGCGGCCTGGTCTGACA-3' |
pEFHAσNS△N20 | F 5'-CGCCGAATTCGGATCaATTTGCTCTTTTACGTTGTGTACTATCAGC-3' R 5'-CGACATCGATGGATCTTATGCGATGCGGCCTGGTCTGACA-3' |
pEFHAσNS△N30 | F 5'-CGCCGAATTCGGATCaAGATTCACGTCAAGCTACTCGCGCT-3' R 5'-CGACATCGATGGATCTTATGCGATGCGGCCTGGTCTGACA-3' |
pEFHAσNS△N40 | F 5'-CGCCGAATTCGGATCaATCTCATTTCCCAGGTTTGGGACGC-3' R 5'-CGACATCGATGGATCTTATGCGATGCGGCCTGGTCTGACA-3' |
pEFHAσNS△N50 | F 5'-CGCCGAATTCGGATCaAAAATGTCTGTCACCGCTGACTGCC-3' R 5'-CGACATCGATGGATCTTATGCGATGCGGCCTGGTCTGACA-3' |
pEFHAσNS△N60 | F 5'-CGCCGAATTCGGATCaAGCAGACCGTACTGGTCCGCCACGTT-3' R 5'-CGACATCGATGGATCTTATGCGATGCGGCCTGGTCTGACA-3' |
Tab.2
Sequences of upstream and downstream primers of each target gene"
Primer | Sequence |
---|---|
μNS | F 5'-GGATTCCCGGGGATCaAATGCGTGTCGGTGTCTCGAGAAA-3' R 5'-GCAGGTCTACGGATCTTATGCGATGCGGCCTGGTCTGACA-3' |
σNS | F 5'-ATCGATAGGGATCCaAATGCGTGTCGGTGTCTCGAGAAA-3' R 5'-CGAGCTCGATGGATCTTATGCGATGCGGCCTGGTCTGACA-3' |
ΔS3-10aa | F 5'-ATCGATAGGGATCCaAGGTACTGGGTCTCAGACCATACTAC-3' R 5'-CGAGCTCGATGGATCTTATGCGATGCGGCCTGGTCTGACA-3' |
ΔS3-20aa | F 5'-ATCGATAGGGATCCaATTTGCTCTTTTACGTTGTGTACTATCAGC-3' R 5'-CGAGCTCGATGGATCTTATGCGATGCGGCCTGGTCTGACA-3' |
ΔS3-30aa | F 5'-ATCGATAGGGATCCaAGATTCACGTCAAGCTACTCGCGCT-3' R 5'-CGAGCTCGATGGATCTTATGCGATGCGGCCTGGTCTGACA-3' |
ΔS3-40aa | F 5'-ATCGATAGGGATCCaATCTCATTTCCCAGGTTTGGGACGC-3' R 5'-CGAGCTCGATGGATCTTATGCGATGCGGCCTGGTCTGACA-3' |
ΔS3-50aa | F 5'-ATCGATAGGGATCCaAAAATGTCTGTCACCGCTGACTGCC-3' R 5'-CGAGCTCGATGGATCTTATGCGATGCGGCCTGGTCTGACA-3' |
ΔS3-60aa | F 5'-ATCGATAGGGATCCaAGCAGACCGTACTGGTCCGCCACGTT-3' R 5'-CGAGCTCGATGGATCTTATGCGATGCGGCCTGGTCTGACA-3' |
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