双氢青蒿素对神经母细胞瘤细胞生长的抑制作用及其机制

doi:10.13481/j.1671-587x.20160215

Abstract

Abstract:

Objective: To explore the inhibitory effect of dihydroartemisinin (DHA) on the growth of neuroblastoma cells, and to clarify the anti-tumor mechanism of DHA.Methods: The experiment was divided into blank control group and DHA groups (the final concentrations of DHA were 0.05, 0.50, 5.00 and 50.00 μmol·L^-1).The proliferation rates of neuroblastoma SH-SY5Y cells after treated with DHA were examined by MTT assay; the changes of cell cycle of SH-SY5Y cells after treated with DHA were examined by flow cytometry;the expression levels of cyclin D₁ and caspase-3 proteins were detected by ELISA and Western blotting methods.Results: The proliferation of SH-SY5Y cells 24, 48, and 72 h after treated with different concentrations of DHA were inhibited.Compared with blank control group, the proliferation rates of SH-SY5Y cells in 0.50, 5.00 and 50.00 μmol·L^-1DHA groups were significantly decreased(P<0.05 or P<0.01).The density of cells was decreased with the increasing of DHA concentration.Compared with blank control group, the percentage of SH-SY5Y cells at SubG₁ phase in 50.00 μmol·L^-1 DHA group was increased(P<0.05), and the percentage of cells at G₀/G₁ phase was increased first then was decreased; otherwise, the percentages of cells at S and G₂/M phase were decreased.Compared with blank control group, the expression level of cyclin D1 protein in 50.00 μmol·L^-1 DHA group was decreased(P<0.05), but the expression level of caspase-3 protein in 50.00 μmol·L^-1 DHA group was increased(P<0.05).Conclusion: DHA could inhibit the proliferation through arresting the cell cycle and inducing the apoptosis of neuroblastoma cells.

Key words: dihydroartemisinin, neuroblastoma cells, cyclin D1, caspase-3

CLC Number:

R739.4

QI Ling, YANG Yang, LIU Yucui, ZHU Tianxin, JIN Song, ZANG Lin, ZHANG Yuying, LYU Peng, XU Ye. Inhibitory effect of dihydroartemisinin on growth of neuroblastoma cells and its mechanism[J].Journal of Jilin University Medicine Edition, 2016, 42(02): 266-270.

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Inhibitory effect of dihydroartemisinin on growth of neuroblastoma cells and its mechanism

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