Journal of Jilin University(Medicine Edition) ›› 2021, Vol. 47 ›› Issue (6): 1407-1414.doi: 10.13481/j.1671-587X.20210609

• Research in basic medicine • Previous Articles     Next Articles

Effect of bisphenol A exposure on mRNA molecule expression profile in ovarian tissue of prepubertal female rats

Xueying ZHANG,Lianwen ZHENG,Yinggang ZOU,Zhenhuan ZHOU()   

  1. Reproductive Medical Center,Second Hospital,Jilin University,Changchun 130041,China
  • Received:2021-05-06 Online:2021-11-28 Published:2021-12-14
  • Contact: Zhenhuan ZHOU E-mail:1063103487@qq.com

Abstract: Objective

To study the effect of bisphenol A (BPA) on the mRNA molecule expression profile in ovarian tissue of the prepubertal female rats, and to explore the potential effect of BPA on the structure and function of ovarian tissue.

Methods

A total of 32 female SD rats were randomly divided into control group and low,middle,and high doses of BPA groups,and there were 8 rats in each group.The rats in low,middle,and high doses of BPA groups were given 50,100,and 200 mg·kg-1 BPA,and the rats in control group were given ethanol-corn oil(1∶100). HE staining was used to observe the morphology of ovarian tissue of the rats in various groups. RNA sequencing (RNA-seq) and bioinformatic analyses were used to predict the differential expression genes and their biological functions between high dose of BPA group and control group. The mRNA expression levels of Hapln3, Nr4a1, Arc, Ccl2, Ccn5, Egr2,and Rab33a in ovarian tissue of the rats in various groups were detected by Real-time fluorescence quantitative PCR (RT-qPCR)method.

Results

The HE staining results showed that compared with control group, there were no obvious abnormalities in ovarian tissue of the rats in low and middle doses of BPA groups. The number of primordial follicles, pre sinus follicles and sinus follicles in ovarian tissue of the rats in high dose of BPA group were decreased, the number of atretic follicles was increased, and the cystic follicles were appeared.The RNA-seq detection results showed that there were 14 differential expression genes in ovarian tissue of the rats in control group and high dose of BPA group. The bioinformatics analysis results showed that 14 mRNA molecules were involved in a variety of biological processes such as reproduction, metabolism and apoptosis, as well as p53, MAPK, PI3K-Akt,and other signal pathways. Compared with control group, the expression levels of Hapln3, Nr4a1, Arc, Ccl2, Ccn5,and Egr2 mRNA in ovarian tissue of the rats in high dose of BPA group were decreased (P<0.05),and the expression level of Rab33a mRNA was increased (P<0.05).

Conclusion

BPA changes the mRNA molecule expression profile in ovarian tissue of the prepubertal female rats, which may potentially influence the structure and function of ovarian tissue.

Key words: bisphenol a, endocrine disrupting chemicals, ovarian tissue, mRNA, rat,SD, prepubertal

CLC Number: 

  • R994.6