人参皂苷Rh2型联合氟化钠对变异链球菌生长及生物膜形成的抑制作用

doi:10.13481/j.1671-587X.20220218

Abstract

Abstract: Objective

To explore the inhibitory effects of ginsenoside Rh2 （G-Rh2） combined with sodium fluoride （NaF） on the growth of Streptococcus mutans and biofilm formation in vitro， and to provide theoretical basis for their application in stomatological clinic.

Methods

The half minimum inhibitory concentrations （MIC₅₀） of G-Rh2 and NaF against Streptococcus mutans were measured by microdilution method；the MIC₅₀ value of G-Rh2 combined with NaF on Streptococcus mutans was measured by chessboard microdilution method，and the fractional inhibitory concentration （FIC） value was calculated to judge the combined effect of these two drugs. When FIC<0.5， it was proved that the two drugs had synergistic inhibitory effect on Streptococcus mutans. The half minimum biofilm inhibition concentrations（MBIC₅₀） of G-Rh2 and NaF against Streptococcus mutans were measured by crystal violet staining test.The experiment was divided into blank control group， MIC₅₀G-Rh2 group， MIC₅₀NaF group and MIC₅₀G-Rh2+MIC₅₀NaF combined group， the growth curve and acid production test were used to evaluate the growth speeds and inhibitory rates of acid production of Streptococcus mutans in each group； the experiment was divided into blank control group， MBIC₅₀G-Rh2 group， MBIC₅₀NaF group and MBIC₅₀G-Rh2+MBIC₅₀NaF combined group，the changes of biofilm formation of Streptococcus mutans and structures were observed by crystal violet staining test and scanning electron microscope in each group.The blank control group did not contain any drugs.

Results

The MIC₅₀ of G-Rh2 and NaF acting on Streptococcus mutans alone were 25.000 and 125.000 mg·L^－1，respectively；the MIC₅₀ of G-Rh2 combined with NaF on Streptococcus mutans were 5.00 and 31.25 mg·L^－1，the FIC index was 0.45 and less than 0.5， which proved that the two drugs had synergistic inhibitory effect on Streptococcus mutans.The MBIC₅₀ of G-Rh2 and NaF were 22.5 and 62.5 mg·L^－1.The growth speed of Streptococcus mutans in MIC₅₀G-Rh2+MIC₅₀NaF combined group was significantly lower than those in blank control group， MIC₅₀G-Rh2 group and MIC₅₀NaF group（P<0.05）.The ΔpH values in MIC₅₀G-Rh2 group，MIC₅₀NaF group and MIC₅₀G-Rh2+MIC₅₀NaF combined group were lower than that in blank control group， and the ΔpH value in MIC₅₀G-Rh2+MIC₅₀NaF combined group was lower than those in MIC₅₀G-Rh2 group and MIC₅₀NaF group（P<0.05）.The inhibition rate of acid production in MIC₅₀G-Rh2+MIC₅₀NaF combined group was higher than those in MIC₅₀G-Rh2 group and MIC₅₀NaF group（P<0.05），and the amount of biofilm formation in MBIC₅₀G-Rh2+MBIC₅₀NaF combined group was significantly lower than those in blank control group，MBIC₅₀G-Rh2 group and MBIC₅₀NaF group （P<0.01）.The scanning electron microscope results showed that the thickness of biofilm， and the mass of extracellular base in MBIC₅₀G-Rh2 group and MBIC₅₀NaF group were decreased， the arrangement of bacteria was loose； while in MBIC₅₀G-Rh2+MBIC₅₀NaF combined group， the biofilm structure was disappeared， the number of bacteria was decreased，and the morphology of bacteria was changed.

Conclusion

The combination of G-Rh2 and NaF has the synergistic inhibitory effect on the growth and biofilm formation of Streptococcus mutans in vitro.

Key words: Streptococcus mutans, Ginsenoside Rh2, Sodium fluoride, Combined drug susceptibility experiments

CLC Number:

R781.1

Yuan FANG,Xiaoxuan LIU,Rui WANG. Inhibitory effects of ginsenoside Rh2 combined with sodium fluoride on growth and biofilm formation of Streptococcus mutans[J].Journal of Jilin University(Medicine Edition), 2022, 48(2): 407-413.

Figures/Tables 6

Fig.1

Fig.2

Tab.1

Fig. 3

Fig. 4

Fig. 5

References 23

1	CONRADS G， ABOUT I. Pathophysiology of dental caries［J］. Monogr Oral Sci， 2018， 27： 1-10.
2	BEDOYA-CORREA C M， RINCÓN RODRÍGUEZ R J， PARADA-SANCHEZ M T. Genomic and phenotypic diversity of Streptococcus mutans［J］. J Oral Biosci， 2019， 61（1）： 22-31.
3	GRIGALAUSKIENĖ R， SLABŠINSKIENĖ E， VASILIAUSKIENĖ I. Biological approach of dental caries management［J］.Stomatologija，2015，17（4）： 107-112.
4	JAIN A， SUPRABHA B S， SHENOY R， et al. Remineralising effectiveness of two fluoride varnishes containing additives： an in vitro study［J］. Oral Health Prev Dent， 2019， 17（4）： 385-393.
5	MANARELLI M M， DELBEM A C B， BÁEZ-QUINTERO L C， et al. Fluoride varnishes containing sodium trimetaphosphate reduce enamel demineralization in vitro ［J］. Acta Odontol Scand， 2017，75（5）： 376-378.
6	YU O Y， MEI M L， ZHAO I S， et al. Remineralisation of enamel with silver diamine fluoride and sodium fluoride［J］. Dent Mater， 2018， 34（12）： e344-e352.
7	ZHAO Y， LI H， WEI S， et al. Antimicrobial effects of chemical compounds isolated from traditional Chinese herbal medicine （TCHM） against drug-resistant bacteria： a review paper［J］. Mini Rev Med Chem， 2019， 19（2）： 125-137.
8	CAO X， YE Q， FAN M， et al. Antimicrobial effects of the ginsenoside Rh2 on monospecies and multispecies cariogenic biofilms［J］.J Appl Microbiol，2019，126（3）： 740-751.
9	KACHUR K， SUNTRES Z E. The antimicrobial properties of ginseng and ginseng extracts［J］. Expert Rev Anti Infect Ther， 2016， 14（1）： 81-94.
10	WEGEHAUPT F， MENGHINI G. Update fluorid ［fluoride update］［J］.Swiss Dent J，2020；130（9）：677-683.
11	DANG M H， JUNG J E， LEE D W， et al. Recovery of acid production in streptococcus mutans biofilms after short-term fluoride treatment［J］.Caries Res，2016，50（4）： 363-371.
12	CHEN X Q， DALIRI E B M， KIM N， et al. Microbial etiology and prevention of dental caries： exploiting natural products to inhibit cariogenic biofilms［J］. Pathogens， 2020， 9（7）： 569.
13	LI X， CHU S， LIN M， et al. Anticancer property of ginsenoside Rh2 from ginseng［J］. Eur J Med Chem， 2020， 203： 112627.
14	IM D S. Pro-resolving effect of ginsenosides as an anti-inflammatory mechanism of panax ginseng［J］. Biomolecules， 2020， 10（3）： 444.
15	YOO H J， JWA S K.Inhibitory effects of β-caryophyllene on Streptococcus mutans biofilm［J］. Arch Oral Biol， 2018， 88： 42-46.
16	LIU S， WEI Y， ZHOU X， et al. Function of alanine racemase in the physiological activity and cariogenicity of Streptococcus mutans［J］. Sci Rep， 2018， 8（1）： 5984.
17	DAMÉ-TEIXEIRA N， DENG D，DO T. Streptococcus mutans transcriptome in the presence of sodium fluoride and sucrose［J］. Arch Oral Biol， 2019， 102： 186-192.
18	LÓPEZ-LÓPEZ A， MIRA A. Shifts in composition and activity of oral biofilms after fluoride exposure［J］. Microb Ecol， 2020， 80（3）： 729-738
19	NAUMOVA E A， WEBER L， PANKRATZ V， et al. Bacterial viability in oral biofilm after tooth brushing with amine fluoride or sodium fluoride［J］. Arch Oral Biol， 2019， 97： 91-96.
20	KITAGAWA H， MIKI-OKA S， MAYANAGI G，et al. Inhibitory effect of resin composite containing S-PRG filler on Streptococcus mutans glucose metabolism［J］. J Dent， 2018， 70： 92-96.
21	ISHIGURO T， MAYANAGI G， AZUMI M， et al. Sodium fluoride and silver diamine fluoride-coated tooth surfaces inhibit bacterial acid production at the bacteria/tooth interface［J］. J Dent， 2019， 84： 30-35.
22	曹茜茜，叶倩琳，周立波，等. 人参皂苷Rh2抑制致龋菌生物膜的实验研究［J］. 口腔医学研究， 2018， 34（12）： 1302-1306.
23	刘迪，张浩，孙宏宇，等. 人参皂苷抑菌活性研究进展［J］.中国病原生物学杂志， 2018， 13（12）：1424-1426.

[1]	QU Tian, ZHANG Hong, ZHANG Zhimin, DU Aobo, SHAO Siqi, YANG Yaoyao, LIU Lu. Differential expression of eriC^F gene in fluoride-resistant strains of Streptococcus mutans and its significance [J]. Journal of Jilin University(Medicine Edition), 2019, 45(03): 551-557.
[2]	SUN Jing-Chun, WANG Chun-Yan, XU Hui, LI An-Sheng. Effect of endoplasmic reticulum stress in renal injury of fluorosis rats [J]. J4, 2009, 35(6): 992-995.

Inhibitory effects of ginsenoside Rh2 combined with sodium fluoride on growth and biofilm formation of Streptococcus mutans

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 23

Related Articles 2

Metrics

Comments

Recommended 0