黄芩素对大鼠神经病理痛的镇痛作用及其机制

doi:10.13481/j.1671-587X.20220307

Abstract

Abstract: Objective

To observe the inhibitory effect of baicalein on the pain behavior caused by chronic sciatic nerve compression in the rats and the regulation effect of glial cell-derived neurotrophic factor （GDNF） expression in the primary sensory neurons， and to clarify the analgesic mechanism of baicalein in the neuropathic pain.

Methods

A total of 48 healthy male SD rats were randomly divided into sham operation group， model group and baicalein group，and there were 16 rats in each group. After anesthesia，the sciatic nerve of the rats in sham operation group was exposed， and the chronic constricting nerve injury of the rats in model group and baicalein group was performed to establish the chronic constriction injury（CCI） models. The rats in baicalein group were injected with baicalein continuously for 7 d after successful modeling. The mechanical withdraw threshold （MWT） and thermal withdrawal latency （TWL） of the rats in various groups were measured at different time points before and after operation to evaluate the pain behavior caused by nerve injury. On the 11th day，6 rats were randomly selected from each group for immunofluorescence staining to determine the number of C-Fos positive neurons in spinal dorsal horn tissue of the rats in various groups. On the 28th day， the fluorescence intensities of GDNF in dosal root ganglion（DRG） of the rats in various groups were detected.

Results

There was no significant difference between MWT and TWL of the rats in various groups before operation （P>0.05）. Compared with sham operation group， the MWT and TWL of the rats in model group and baicalein group were decreased significantly 1 d after operation （P<0.05）， and they were increased in baicalein group 3 d after administration （P>0.05）. Compared with sham operation group， the number of c-Fos positive neurons in spinal dorsal horn tissue of the rats in model group was increased （P<0.05）； compared with model group， the number of c-Fos positive neurons in spinal dorsal horn tissue of the rats in baicalein group was decreased （P<0.05）. Compared with sham operation group， the fluorescence intensity of GDNF in DRG of the rats in model group was decreased （P<0.05）；compared with model group， and the fluorescence intensity of GDNF in DRG of the rats in baicalein group was increased （P<0.05）.

Conclusion

Baicalein has the analgesic effect on neuropathic pain of the rats.The GDNF in primary sensory neurons is involved in the neuropathic pain caused by sciatic nerve injury. The analgesic effect of baicalein on the neuropathic pain may be related to its regulation of GDNF expression.

Key words: Baicalein, Neuropathic pain, Glial cell line-derived neurotropic factor, Mechenaical withdral threshold, Thermal withdrawal latency

CLC Number:

R741.02

Xiaohua LIU,Bingying FANG,Man HAN,Haifa QIAO,Yuanwang YU. Analgesic effect of baicalein on neuropathic pain of rats and its mechanism[J].Journal of Jilin University(Medicine Edition), 2022, 48(3): 600-605.

Figures/Tables 4

References 19

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Analgesic effect of baicalein on neuropathic pain of rats and its mechanism

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