Abstract:

Objective To investigate the effect of arctigenin on the metabolites in serum of the cerebral ischemia-reperfusion injury model rats， and to clarify the molecular mechanism of arctigenin for protection of cerebral ischemia-reperfusion injury. Methods A total of 18 SPF SD rats were randomly divided into sham operation group， model group and arctigenin group， with six rats in each group. The rats in arctigenin group were administered with 100 mg·kg^－1 arctigenin by gavage，and the rats in sham operation group and model group were administrated with saline at the same volume lasted for 14 d， then the cerebral ischemia-reperfusion rat models were established by improved line embolization. After 24 h of reperfusion， the neurological deficit scores of the rats in various groups were assessed， HE staining was used to observe the pathomorphology of ischemic penumbra of the rats in variuos groups， and metabolomics technique was used to detect the metabolite levels in serum of the rats in various groups. Results Compared with sham operation group， the score of neurological deficit of the rats in model group was significantly increased （P<0.05）， the morphology of brain tissue was significantly changed and a small amount of glialcell hyperplasia was seen，the levels of 14 kinds of serum metabolites including 3，3'，4'，5-tetrahydroxysilbene and docosahexaenoic acid （DHA） and so on were significantly decreased （P<0.05）， and the levels of 12 kinds of serum metabolites including carnosine and so on were significantly increased （P<0.05）； compared with model group， the score of neurological deficit of the rats in arctigenin group was significantly decreased （P<0.05）， neuronal injury was improved， the levels of 14 kinds of serum metabolites including 3，3'，4'，5-tetrahydroxysilbene and DHA and so on were significantly increased （P<0.05）， and the levels of 12 kinds of serum metabolites including carnosine and so on were significantly decreased （P<0.05）. Conclusion Arctigenin may play an anti-cerebral ischemia-reperfusion role by regulating the metabolites such as DHA， 3，3'，4'，5-tetrahydroxysilbene and carnosine involved in the amino acid metabolism pathway such as histidine metabolism and arginine and proline metabolism.

Key words: Arctigenin, Cerebral Ischemia-reperfusion, Metabolomics, Neurological deficit score