Abstract:

Objective
To study the effects of ethanol extract from Java Brucea Fruit on platelet-derived growth factor receptor β (PDGFRβ) mediated cell migration and to obtain the valuable messages on the characteristics of its active ingredient. 
Methods   PAE cells was transfected with the vector expressing human PDGFRβ with Transfectom 2000;After screening by G418 resisitance,RT-PCR was used to monitor the expression of PDGFRβ in the cells;Wound healing of the cells was used to examine the lowest consistency of PDGFBB and inhibitory effect of ethanol extract of Java Brucea Fruit on cell migration after restoring 24 h.Results Human PDGFRβ was stably expressed in PAE cells transfected with the expressing vector.The lowest consistency of exogenous PDGFBB which promoted PDGFRβ mediated cell migration was 10 μg·L^-1.70% ethanol extract of Java Brucea Fruit which strongly inhibited PDGFRβ　mediated cell migration was dose-dependent（P<0.05）,50% and 25% ethanol extract from Java Brucea Fruit (≤10  μg·L^-1) had the weaker inhibitory effect, ethanol extract from Java Brucea Fruit (>10 μg·L^-1 )  mainly caused the death of the cell. Conclusion Ethanol extract of Java Brucea Fruit has a strongly inhibitory effect on the PDGFRβ mediated cell migration which could play a major role in its effects against metastasis of malignant tumor,the active ingredients of it could be more dissolvable in the 70% ethanol.

Key words: Java Brucea Fruit extract;malignant tumor;platelet-derived growth factor receptor;cell migration