Abstract:

To investigate the association between polymorphism of protein C inhibitor（PCI）gene G10877T and male infertility,and provide theoretical basis for treatment of male infertility.Methods PCR and sequencing technique were applied to detect PCI gene G10877 T polymorphism in 53 normal control and 102 male　 infertility.Results There were three genotypes of wild type（G/C）,hybridization mutation（G/T）and pure mutation （T/T）.The analysis of sequencing indicated that in sperums of a proportion of the male infertile patients,TGG in PCI gene G10877T mutated into TGT.The contrast of BLASTB indicated that this mutation made Trp in 271 position change into Cys.Compared with control group,TT genotypic frequency and T allelic  frequency in male infertility group had significant differences（P<0.05).
Conclusion
 PCI gene G10877 T polymorphism is related to  male infertility,and the  polymorphism of PCI gene increases the risk of male infertility morbidity.

Key words: male infertility;protein C inhibitor;G10877 T polymorphism