Abstract: Objective:To establish the pancreatic carcinoma models of rats with dimethybenzanthracene(DMBA) embedding method, to discuss the treatment effect of Da Huang Mu Dan Tang (DHMDT) in the rats with pancreatic carcinoma and the protective effects on the liver and kidney function, and to clarify the mechanisms. Methods:Sixty-five SD male rats were divided into blank control (n=10) and modeling group (n=55). DMBA was embedded in the capsule of pancreas to establish the pancreatic carcinoma models of rats. After 3 months, 50 successfully modeling rats were randomly divided into model, 5-FU (intravennous injection, 12 mg·kg^-1), low, middle and high doses of DHMDT (intragastric administration, 20,40 and 80 mg·kg^-1) groups. After 10 weeks of administration, the levels of alanine aminotransferase(ALT), aspartate transaminase(AST), urea nitrogen(BUN), creatinine(Cr) and total bilirubin(TBiL) in serum of the rats in various groups were measured; the weights and the inhibitory rates of the pancreatic tumor were detected; TUNEL method was used to analyze the apoptotic rates of tumor tissue; the expression levels of SST2R, Bax and Bcl-2 genes in pancreatic tumor tissue of the rats were detected by Q-PCR method. Results:Compared with model group, after treated for 10 weeks, the levels of ALT, AST, BUN, Cr and TBiL in serum of the rats in low, middle and high doses of DHMDT groups were significantly decreased (P<0.05 or P<0.01); the weights of tumor of the rats in middle and high doses of DHMDT groups were decreased (P<0.05 or P<0.01); the apoptotic rates of the cells in pancreatic tumor tissue of the rats in low, middle and high doses of DHMDT groups were increased (P<0.05 or P<0.01); the expression levels of SST2R gene in pancreatic tumor tissue of the rats in low, middle and high doses of DHMDT groups were significantly increased (P<0.05 or P<0.01); the expression levels of Bax gene in pancreatic tumor tissue of the rats in middle and high doses of DHMDT groups were significantly increased(P<0.05 or P<0.01), while the expression levels of Bcl-2 in pancreatic tumor tissue of the rats were significantly decreased (P<0.05). Conclusion:DHMDT has the treatment effect in the rats with pancreatic carcinoma and can promote the apoptosis of tumor cells;DHMDT can protect the liver and kidney function of the rats with pancreatic carcinoma.

Key words: Da Huang Mu Dan Tang, dimethybenzanthracene, apoptosis, pancreas neoplasms