Abstract: Objective To clone aquaporin 8 (AQP8) cDNA of testis in Wistar rats and construct the eucaryotic expression vector of enhance green fluorescent protein (EGFP) and AQP8 fusion protein.Methods The full sequence of AQP8 was fished by RT-PCR and sequenced, then it was recombined in the downstream of the pEGFP-C1 gene. Results The sequence of AQP8 cDNA of Wistar rat was homology with NM_019158（access number logged in GenBank），but there were 4 different bases：P135-137 of NM_019158 partly were C，A and G，but the sequencing result was absence；P311 of NM_019158 was A，but the sequencing result was G. The recombinant pEGFP-C1-AQP8 was identified by enzyme digestion. Conclusion The recombinant pEGFP-C1-AQP8 is constructed successfully.

Key words: fusion protein, green fluorescent protein, testes, rats