Abstract:

Objective
To explore the lead-expelling effect of Quqian Yizhi Health Oral Liquid (QYHOL) in lead-poisoning rats,and provide experimental basis for prevention and therapy of lead poisoning. Methods 72 SD rats were randomly divided into six groups. One group was negative control group only gavaging with distilled water,and others were used to establish lead poisoning models by gavaging 2.0% lead acetate  for three weeks.Positive control group was gavaged with distilled water,EDTA group was treated with 0.5% EDTA by abdominal cavity injection and the last three groups were gavaged with low,medium and high-dose QYHOL(1.25,2.50 and 5.00 mg?kg-1 respectively) for three weeks.At the end of the experiment,all rats were killed and the samples of blood,livers,kidneys and femurs were collected.The lead level in each sample was measured by hydride generated-atomic absorption spectrographic method.The pathological  changes of tissue sections of liver,kidney and other organs stained with HE were observed by light microscope.Results Compared with positive control group,the lead levels of rat blood samples,livers,kidneys and femurs were markedly  decreased after treated with QYHOL with three dosages.Among three dosage QYHOL groups,there were no significant differences in the decreasing   percentage of  lead levels in blood and kidney tissues (P>0.05).The decreasing  percentage of lead levels  in liver tissues in low-dosage group was higher than those in high-dosage and EDTA groups(P<0.05 or P<0.01).High-dosage QYHOL markedly decreased the lead levels in femur tissues,and compared with low-,mid-dosage and EDTA groups,there were significant differences(P< 0.01).All pathological sections with HE staining did not reveal abnormal structures by light microscope.Conclusion  QYHOL has safty and effective role on lead-poisoning rats.
         
            

Key words: lead-poisoning/drug therapy;rat;lead-expelling;animal,laboratory