Abstract:

Abstract:Objective To observe the influence of  aprotinin in   ultrastructural changes of liver tissues in mice with experimental acute liver injury and   explore its  protective effect on  liver tissues.  Methods The mouse medels with carbon tetrachloride (CCl₄) liver injury were established and the mice were divided into normal control group,model group,ganlixin injection groups and low,  middle  and high dose aprotinin groups. The tissue specimens were obtained from various groups,uranium oxide-acetate-citric acid-lead double staining was used to observe the changes of liver tissues under TEM.  Results In model group,  the liver cells were  swelled with pyknotic nucleus,reduced intracytoplasmic rough endoplasmic reticulum,pyknotic mitochondria,and cavitation. In  low  dose aprotinin group,the  individual cells were  pyknotic with a few rough endoplasmic reticulum and lysosomes in liver cells,timid Endoluminal microvilli disappeared.In middle  dose  aprotinin group, the size of  liver cells was a little larger,the liver cell nucleolar was evident with intracytoplasmic rough endoplasmic reticulum, mitochondria,numerous glycogen accumulation and more timid Endoluminal microvilli. In high dose aprotinin group,the liver cell structures were similar to those in control . The ultrasturctural changes in ganlixin injection group were similar to those in middle dose aprotinin group.  Conclusion The intraperitoneal injection of aprotinin has significantly protective effect on the ultrastructures of liver tissues in acute liver injury mouse models.With the increase of aprotinin,the ultrastructural changes of  liver tissues are markedly reduced.

Key words: aprotinin;carbon tetrachloride;acute liver injury;mice;electron microscope