二甲双胍通过PI3K/AKT/GSK3β信号通路对肺癌A549细胞增殖的影响

doi:10.13481/j.1671-587X.20210313

Abstract

Abstract: Objective

To investigate the effect of metformin on the proliferation of the non-small cell lung cancer A549 cells ，and to clarify its possible mechanism.

Methods

The A549 cells were divided into control group and different concentrations （1， 2， 4， 8， 16 and 32 mmol·L^－1） of metformin groups.MTT method was used to detect the proliferation rates of A549 cells in various groups after treated for 24， 48 and 72 h； clone formation experiment was used to detect the clone formation number of A549 cells in various groups； cell scratch test was used to detect the migration rates of A549 cells in various groups； RT-PCR method was used to detect the expression levels of phosphoinositide-3-kinase （PI3K）， protein kinase B （AKT）， and glycogen-synthase kinase-3β （GSK3β） mRNA in the A549 cells in various groups； Western blotting method was used to detect the expression levels of PI3K， PI3K（p-PI3K）， AKT， phosphorylated AKT（p-AKT）， GSK3β ，and phosphorylated GSK3β （p-GSK3β） proteins in the A549 cells in various groups.

Results

Compared with control group， the proliferation rates of lung cancer A549 cells in different concentrations of metformin groups after treated for 24， 48 and 72 h were decreased （P<0.05）； compared with control group， the number of clone formation in the A549 cells in 2 and 8 mmol·L^－1 metformin groups was decreased （P<0.05）； compared with control group， the migration rates of the A549 cells in 2 and 8 mmol·L^－1 metformin groups were decreased （P<0.05）； compared with control group， the expression levels of PI3K， AKT and GSK3β mRNA in the A549 cells in 2 and 8 mmol·L^－1 metformin groups were significantly decreased （P<0.05）； compared with control group， the expression levels of PI3K， p-PI3K， p-AKT， and p-GSK3β proteins in the A549 cells in 2 and 8 mmol·L^－1 metformin groups were significantly decreased （P<0.05）.

Conclusion

Metformin can inhibit the proliferation and migration of lung cancer A549 cells， and its mechanism may be related to the inhibition of PI3K/AKT/GSK3β signaling pathway.

Key words: lung cancer, metformin, proliferation, migration, PI3K/AKT/GSK3β signaling pathway

CLC Number:

R734.2

Chunqing MU, Lei ZHOU, Nan ZHAO, Hong WANG, Zhi LI. Effect of metformin on proliferation of lung cancer A549 cells through PI3K/AKT/GSK3β signaling pathway[J].Journal of Jilin University(Medicine Edition), 2021, 47(3): 637-643.

Figures/Tables 8

Tab. 1

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Group		Proliferation rate of A549 cells
Group	（t/d） 24		48	72
Control		100.00±0.00	100.00±0.00	100.00±0.00
Metformin (mmol·L^－1)
1		96.95±1.88^*	91.09±0.41^*	91.54±4.25^*
2		91.80±1.23^*	87.87±3.46^*	81.91±1.00^*
4		87.01±8.19^*	84.13±1.06^*	76.24±0.22^*
8		84.46±1.61^*	74.79±0.41^*	60.32±2.98^*
16		81.90±6.79^*	67.20±2.00^*	52.99±2.13^*
32		79.60±1.37^*	57.04±3.77^*	22.68±0.30^*

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Effect of metformin on proliferation of lung cancer A549 cells through PI3K/AKT/GSK3β signaling pathway

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