脂肪间充质干细胞对卵巢早衰模型大鼠的治疗作用及其机制

doi:10.13481/j.1671-587X.20220313

Abstract

Abstract: Objective

To explore the biological characteristics of adipose-derived mesenchymal stem cells （ADMSCs）， and to clarify the therapeutic effect of ADMSCs in the premature ovarian failure （POF） model rats.

Methods

The ADMSCs from SD rats were isolated and cultured， and the morphology of cells was observed under optical microscope. The osteogenic and adipogenic differentiations of ADMSCs were induced by osteogenic and adipogenic cell induction reagents. The expressions of surface antigen markers CD44， CD90，and CD45 of ADMSCs were detected by flow cytometry.The growth of ADMSCs was determined by CCK-8 assay and the growth curves were drawn. The models of PDF were established by intraperitoneal injection of cisplatin.Thirty SD rats were randomly divided into normal control group （n=10） and modeling group （n=20）. The rats in modeling group were randomly divided into model group and ADMSCs treatment group， and there were 10 rats in each group.The rats in ADMSCs treatment group were injected with allogeneic ADMSCs through the tail vein every 3 d； the rats in normal control group and model group were injected with normal saline every 3 d through the tail vein. The body weights of the rats in various groups were detected， and the levels of estradiol （E2）， follicle stimulating hormone （FSH）， and luteinizing hormone （LH） in serum of the rats in various groups were detected by ELISA mehod. The pathomorphology of ovary tissue of the rats in various groups was observed by HE staining.

Results

The ADMSCs were adherent growth cells， which grew vigorously and could differentiate into the osteogenic and adipogenic cells. The expressions of CD44 and CD90 in the ADMSCs were highly， and the expression rates were 75.85% and 59.71%； the expression of CD45 was low， and the expression rate of 39.12%. Compared with normal control group， the body weight of the rats in model group was significantly decreased （P<0.05）； the serum E2 level was significantly decreased （P<0.05）， and the levels of FSH and LH were significantly increased （P<0.05）， lacking of follicles at all levels， and the number of atretic follicles was increased. Compared with model group， the serum E2 level of the rats in ADMSCs treatment group was increased （P<0.05）， the FSH and LH levels were decreased （P<0.05）； the number of follicles was increased，and the number of atretic follicles was decreased.

Conclusion

ADMSCs can grow and reproduce in vitro， and have strong growth and proliferation abilities and multi-directional differentiation abilities； ADMSCs can improve the serum E2 level and ovarian function of the POF model rats， and have a certain therapeutic effect in the POF model rats.

Key words: Adipose-derived mesenchymal stem cells, Cell differentiation, Cell proliferation, Premature ovarian failure, Ovarian function

CLC Number:

R711

Peiyu YAN,Aichen ZHANG,Hong ZHANG,Yang LI,Mengmeng ZHANG,Mengze LUO,Ying PAN. Therapeutic effect of adipose-derived mesenchymal stem cells on premature ovarian failure model rats and its mechanism[J].Journal of Jilin University(Medicine Edition), 2022, 48(3): 648-656.

Figures/Tables 10

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References 25

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Group	Body weight (m/g)		E₂[ρ_B/(ng·L^－1)]	FSH[λ_B/（IU·L^－1）]	LH[ρ_B/(ng·L^－1)]
Group	Before treatment	After treatment	E₂[ρ_B/(ng·L^－1)]	FSH[λ_B/（IU·L^－1）]	LH[ρ_B/(ng·L^－1)]
Normal control	269.98±1.29	276.59±4.65	49.85±14.29	3.47±0.86	306.61±216.33
Model	167.18±15.91	167.62±0.47	32.56±9.92^*	4.20±0.36^*	694.55±247.87^*
ADMSCs treatment	163.41±21.70	168.52±3.64	45.69±9.13^△	3.57±0.69^△	400.21±218.80^△

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Therapeutic effect of adipose-derived mesenchymal stem cells on premature ovarian failure model rats and its mechanism

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