吉林大学学报(工学版) ›› 2017, Vol. 47 ›› Issue (1): 332-336.doi: 10.13229/j.cnki.jdxbgxb201701048

• 论文 • 上一篇    

基于抗氧化的大豆异黄酮对PC12细胞的神经保护作用

徐梦蕾1, 高宇2, 刘静波1, 熊晋锋3, 赵颂宁1, 黄延军1   

  1. 1.吉林大学 营养与功能食品实验室,长春 130062;
    2.吉林农业大学 农学院,长春 130118;
    3.长春生物制品研究所有限责任公司,长春 130012
  • 收稿日期:2016-01-11 出版日期:2017-01-20 发布日期:2017-01-20
  • 通讯作者: 刘静波(1962-),女,教授,博士生导师.研究方向:营养与功能食品.E-mail:ljb168@sohu.com
  • 作者简介:徐梦蕾(1988-),女,博士研究生.研究方向:营养与功能食品.E-mail:xuml13@mails.jlu.edu.cn
  • 基金资助:
    国家科技支撑计划项目(2012BAD33B03); 吉林大学研究生创新基金项目(2015052); 吉林农业大学科研启动基金项目(2015010).

Neuroprotective effects of soy isoflavones on PC12 cells based on antioxidation

XU Meng-lei1, GAO Yu2, LIU Jing-bo1, XIONG Jin-feng3, ZHAO Song-ning1, HUANG Yan-jun1   

  1. 1.Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, China;
    2.College of Agriculture, Jilin Agricultural University, Changchun 130018, China;
    3.Changchun Institute of Biological Products Co., Ltd., Changchun 130012, China
  • Received:2016-01-11 Online:2017-01-20 Published:2017-01-20

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摘要： 实验研究了20.00 μg/mL的单一染料木黄酮(GEN)、大豆黄素(DAI)及其混合溶液(GEN-DAI)对PC12细胞的存活率、形态、线粒体膜电位以及超氧化物歧化酶活力和抑制率的影响。结果表明:3种溶液对上述指标均没有显著影响,但GEN提高了活性氧水平,相对荧光强度和丙二醛含量有所升高;DAI和GEN-DAI溶液显著地降低了乳酸脱氢酶漏出率,谷胱甘肽含量分别增加到(297.27±9.47) μmol/(g·prot)、(269.90±0.00) μmol/(g·prot);GEN-DAI混合溶液综合增强了胆碱系统的代谢,提高了乙酰胆碱含量。GEN-DAI混合溶液比两种单一组分溶液能够更有效地提高PC12细胞抗氧化系统活力。

关键词: 食品加工技术, PC12细胞, 大豆异黄酮, 抗氧化, 神经保护作用, 染料木黄酮, 大豆黄素

Abstract: Experiment results show that when PC12 cells were exposed to 20.00 μg/mL genistein (GEN), daidzein (DAI) or their combination (GEN-DAI), the cells' viability rates, mophologies, mitochondrial membrane potential, superoxide dismutase activities and inhibition rates were not changed. However, different changes of PC12 cells were observed under different treatments. The reactive oxygen species level, the relative fluorescence intensity and malondialdehyde of PC12 cells were increased by GEN. The leakage rate of lactic dehydrogenase was decreased in DAI and in GEN-DAI combination treatments, the glutathione contents were increased to (297.27±9.47) μmol/(g·prot) and (269.90±00) μmol/(g·prot) respectively. The acetylcholine content in culture medium and metabolism of cholinergic system was increased in GEN-DAI treatment. GEN-DAI combination treatment can more significantly increase the activity of the antioxidant system of PC12 cells than GEN and DAI treatments.

Key words: food processing technology, PC12 cells, soy isoflavones, antioxidant, neuroprotective effect, genistein, daidzein

中图分类号: 

  • TS201
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