吉林大学学报(工学版) ›› 2014, Vol. 44 ›› Issue (2): 573-578.doi: 10.13229/j.cnki.jdxbgxb201402046

• 论文 • 上一篇    下一篇

高压脉冲电场对淀粉和肌原纤维蛋白混合凝胶强度的影响

金声琅1,2, 王莹3, 殷涌光2   

  1. 1. 黄山学院 旅游学院, 安徽 黄山 245021;
    2. 吉林大学 生物与农业工程学院, 长春 130022;
    3. 青岛农业大学食品科学与工程学院, 山东 青岛 266109
  • 收稿日期:2013-01-06 出版日期:2014-02-01 发布日期:2014-02-01
  • 通讯作者: 殷涌光（1949- ），男，教授，博士生导师.研究方向：农产品加工及设备.E-mail：biofood@jlu.edu.cn E-mail:biofood@jlu.edu.cn
  • 作者简介:金声琅（1980- ），男，副教授，博士研究生.研究方向：农产品加工及设备.E-mail：jinshenglang@163.com
  • 基金资助:

    安徽省高等学校省级自然科学研究项目（KJ2011B165）.

Effect of high intensity pulsed electric field pretreatment on elasticity of starch-myofibril protein mixed gelatin

JIN Sheng-lang1,2, WANG Ying3, YIN Yong-guang2   

  1. 1. Tourism College, Huangshan University, Huangshan 245021, China;
    2. College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, China;
    3. School of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China
  • Received:2013-01-06 Online:2014-02-01 Published:2014-02-01

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摘要：

以肌原纤维蛋白为研究对象，将不同质量分数的葛根淀粉添加其中，混合溶液经高压脉冲电场预处理后，再经水浴加热处理形成混合凝胶，测定其凝胶强度。单因素试验和正交分析试验表明，在场强30 kV/cm、脉冲数6的条件下，经高压脉冲电场预处理后，肌原纤维蛋白（质量分数5%）和葛根淀粉（质量分数5%）混合溶液在80℃水浴加热30 min，可以形成较好的混合凝胶。各因素对混合凝胶强度影响程度由大至小依次为电场强度、脉冲数、葛根淀粉质量分数。研究结果表明，高压脉冲电场预处理能够促进肌原纤维蛋白和葛根粉形成良好的混合凝胶。

关键词: 食品加工技术, 高压脉冲电场, 肌原纤维蛋白, 葛根淀粉, 凝胶

Abstract:

Pueraria starch of different concentration was added to myofibril protein to form mixed gels by high intensity pulsed electric fields and water bath treatment. Gelling properties were determined. The effects of the electric intensity, pulse number and mass fraction of pueraria starch were studied. It was demonstrated by single factor experiment and orthogonal analysis experiment, that mix solutions of myofibril protein (5%) and pueraria starch (5%) could acquire favorable mixed gels by the high intensity pulsed electric fields pretreatment and water bath of 80℃ for 30 min when electric intensity was 30 kV/cm and pulse number was 6. The influence sequence of factors on mixed gels was electric intensity, pulse number and mass fraction of pueraria starch. Study results indicate that the high intensity pulsed electric field pretreatment could promote myofibril protein and pueraria starch to form favorable mixed gels.

Key words: food processing technology, high intensity pulsed electric fields, myofibril protein, pueraria starch, gel

中图分类号: 

  • TS201.7

[1] 孔保华, 王宇, 夏秀芳, 等.加热温度对猪肉肌原纤维蛋白凝胶特性的影响[J].食品科学, 2011, 32(5):50-54. Kong Bao-hua, Wang Yu, Xia Xiu-fang, et al. Influence of heating temperature on gel properties of porcine myofibrillar protein[J]. Food Science, 2011, 32(5): 50-54.

[2] Park D, Xiong You-ling. Oxidative modification of amino acids in porcine myofibrillar protein isolates exposed to three oxidizing systems[J]. Food Chemistry, 2007, 103(2): 607-611.

[3] 陈从贵, 李珂昕, 马力量, 等.超高压对含有琼脂猪肉凝胶特性影响的试验[J].农业工程学报, 2008, 24(10):217-221. Chen Cong-gui, Li Ke-xin, Ma Li-liang, et al. Effects of ultra high pressure on the properties of porkmeat gels containing agar[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2008, 24(10): 217-221.

[4] Jiang Qian-qian, Xu Xue-ming, Jin Zheng-yu, et al. Physico-chemical properties of rice starch gels: effect of different heat treatments[J].Journal of Food Engineering, 2011, 107(3/4): 353-357.

[5] Ribotta P D, Colombo A, Rosell C M.Enzymatic modifications of pea protein and its application in protein-cassava and corn starch gels[J]. Food Hydrocolloids, 2012, 27(1): 185-190.

[6] Chin K B, Go M Y, Xiong You-ling. Konjac flour improved textural and water retention properties of transglutaminase-mediated, heat-induced porcine myofibrillar protein gel: effect of salt level and transglutaminase incubation[J]. Meat Science, 2009, 81(3): 565-572.

[7] Shim J, Mulvaney S J. Effect of heating temperature, pH, concentration and starch/whey protein ratio on the viscoelastic properties of corn starch/whey protein mixed gels[J]. Journal of the Science of Food and Agriculture, 2001, 81(8):706-717.

[8] Verbeken D, Thas O, Dewettinck K. Textural properties of gelled dairy desserts containing k-carrageenan and starch[J]. Food Hydrocolloids, 2004, 18(5): 817-823.

[9] 赵瑾, 杨瑞金, 赵伟, 等.高压脉冲电场对鲜榨梨汁的杀菌效果及其对产品品质的影响[J].农业工程学报, 2008, 24(6):239-244. Zhao Jin, Yang Rui-jin, Zhao Wei, et al. Effect of pulsed electric fields on inactivation of microbe and quality of freshly-squeezed pear juice[J].Transactions of the CSAE, 2008, 24(6): 239-244.

[10] 崔晓美, 杨瑞金, 赵伟, 等.高压脉冲电场对石榴汁杀菌的研究[J].农业工程学报, 2007, 23(3):252-256. Cui Xiao-mei, Yang Rui-jin, Zhao Wei, et al. Pasteurization of pomegranate juice using high-intensity pulsed electric fields[J]. Transactions of the CSAE, 2007, 23(3): 252-256.

[11] 殷涌光, 樊向东, 刘凤霞, 等.用高压脉冲电场技术快速提取苹果渣果胶[J].吉林大学学报:工学版, 2009, 39(5):1224-1228. Yin Yong-guang, Fan Xiang-dong, Liu Feng-xia, et al. Fast extraction of pectin from apple pomace by high intensity pulsed electric field[J]. Journal of Jilin University: Engineering and Technology Edition, 2009, 39(5): 1224-1228.

[12] 金声琅, 殷涌光.高压脉冲电场辅助提取番茄皮渣的番茄红素[J].农业工程学报, 2010, 26(9):368-373. Jin Sheng-lang, Yin Yong-guang. High intensity pulsed electric fields assisted extraction of lycopene from tomato residual[J]. Transactions of the CSAE, 2010, 26(9): 368-373.

[13] 李迎秋, 陈正行.高压脉冲电场对大豆分离蛋白功能性质的影响[J].农业工程学报, 2006, 22(8):194-198. Li Ying-qiu, Chen Zheng-xing. Effect of high intensity pulsed electric field on the functional properties of protein isolated from soybean[J]. Transactions of the CSAE, 2006, 22(8): 194-198.

[14] 赵武奇, 殷涌光, 关伟, 等.高压脉冲电场杀菌系统设计与试验[J].农业机械学报, 2002, 33(3):67-69. Zhao Wu-qi, Yin Yong-guang, Guan Wei, et al. Experimental study on a food pasteurization system by using a high voltage pulsed field[J]. Transactions of the CSAM, 2002, 33(3): 67-69.

[15] Pialr Trespalacios, Reyes Pla. Simultaneous application of transglutaminase and high pressure to improve functional properties of chicken meat gels[J].Food Chemistry, 2007, 100:264-272.

[16] 王岩, 王存堂, 蒋继丰, 等.离子强度和温度对乳清蛋白凝胶的影响[J].食品科学, 2010, 31(1):123-126. Wang Yan, Wang Cun-tang, Jiang Ji-feng, et al. Effects of ionic strength and temperature on whey protein gelation[J]. Food Science, 2010, 31(1): 123-126.

[17] 陆剑锋, 邵明栓, 林琳, 等.卡拉胶和超高压对鱼糜凝胶性质的影响[J].农业机械学报, 2011, 42(12):164-170. Lu Jian-feng, Shao Ming-shuan, Lin Lin, et al. Effects of ultra-high pressure and carrageenan on gelling properties of surimi from silver carp, hypophthalmichthys molitrix[J]. Transactions of the CSAM, 2011, 42(12): 164-170.

[18] Lange R, Frank J, Saldana J L, et al.Fourth derivative UV-spectroscopy of proteins under high pressure I.Factors affecting the fourth derivative spectrum of aromatic amino acids[J]. European Biophysics Journal, 1996, 24(5):277-283.

[19] Mombelli E, Afshar M, Fusi p Mariani, et al.The role of phenylalanine 31 in maintaining the conformational stability of ribonuclease P2 from Sulfolobus solfataricus under extreme conditions of temperature and pressure[J].Biochemistry, 1997, 36(29):8733-8742.

[20] 张铁华, 殷涌光.高压脉冲电场对食品中生物大分子的影响[J].食品科技, 2007, 189(7):16-20. Zhang Tie-hua, Yin Yong-guang. Effect of high-intensity pulsed electric fields(PEF) on food biomacromolecule[J]. Food Science and Technology, 2007, 189(7):16-20.

[21] 张铁华, 殷涌光, 刘静波.高压脉冲电场(PEF)对蛋清蛋白功能特性的影响[J].食品科学, 2007, 28(9):98-102. Zhang Tie-hua, Yin Yong-guang, Liu Jing-bo. Effects of high intensity pulsed electric fields on functional properties of egg albumen[J]. Food Science, 2007, 28(9): 98-102.

[22] 金声琅, 殷涌光, 王莹. 脉冲电场协同加热对乳清蛋白凝胶质构特性的影响[J].农业机械学报, 2013, 44(1):142-146. Jin Sheng-lang, Yin Yong-guang, Wang Ying. Effects of combined pulsed electric field and heat treatment on texture characteristics of whey protein gels[J]. Transactions of the CSAM, 2013, 44(1): 142-146.
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