吉林大学学报(工学版) ›› 2017, Vol. 47 ›› Issue (1): 332-336.doi: 10.13229/j.cnki.jdxbgxb201701048

• 论文 • 上一篇    

基于抗氧化的大豆异黄酮对PC12细胞的神经保护作用

徐梦蕾1, 高宇2, 刘静波1, 熊晋锋3, 赵颂宁1, 黄延军1   

  1. 1.吉林大学 营养与功能食品实验室,长春 130062;
    2.吉林农业大学 农学院,长春 130118;
    3.长春生物制品研究所有限责任公司,长春 130012
  • 收稿日期:2016-01-11 出版日期:2017-01-20 发布日期:2017-01-20
  • 通讯作者: 刘静波(1962-),女,教授,博士生导师.研究方向:营养与功能食品.E-mail:ljb168@sohu.com
  • 作者简介:徐梦蕾(1988-),女,博士研究生.研究方向:营养与功能食品.E-mail:xuml13@mails.jlu.edu.cn
  • 基金资助:
    国家科技支撑计划项目(2012BAD33B03); 吉林大学研究生创新基金项目(2015052); 吉林农业大学科研启动基金项目(2015010).

Neuroprotective effects of soy isoflavones on PC12 cells based on antioxidation

XU Meng-lei1, GAO Yu2, LIU Jing-bo1, XIONG Jin-feng3, ZHAO Song-ning1, HUANG Yan-jun1   

  1. 1.Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, China;
    2.College of Agriculture, Jilin Agricultural University, Changchun 130018, China;
    3.Changchun Institute of Biological Products Co., Ltd., Changchun 130012, China
  • Received:2016-01-11 Online:2017-01-20 Published:2017-01-20

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摘要： 实验研究了20.00 μg/mL的单一染料木黄酮(GEN)、大豆黄素(DAI)及其混合溶液(GEN-DAI)对PC12细胞的存活率、形态、线粒体膜电位以及超氧化物歧化酶活力和抑制率的影响。结果表明:3种溶液对上述指标均没有显著影响,但GEN提高了活性氧水平,相对荧光强度和丙二醛含量有所升高;DAI和GEN-DAI溶液显著地降低了乳酸脱氢酶漏出率,谷胱甘肽含量分别增加到(297.27±9.47) μmol/(g·prot)、(269.90±0.00) μmol/(g·prot);GEN-DAI混合溶液综合增强了胆碱系统的代谢,提高了乙酰胆碱含量。GEN-DAI混合溶液比两种单一组分溶液能够更有效地提高PC12细胞抗氧化系统活力。

关键词: 食品加工技术, PC12细胞, 大豆异黄酮, 抗氧化, 神经保护作用, 染料木黄酮, 大豆黄素

Abstract: Experiment results show that when PC12 cells were exposed to 20.00 μg/mL genistein (GEN), daidzein (DAI) or their combination (GEN-DAI), the cells' viability rates, mophologies, mitochondrial membrane potential, superoxide dismutase activities and inhibition rates were not changed. However, different changes of PC12 cells were observed under different treatments. The reactive oxygen species level, the relative fluorescence intensity and malondialdehyde of PC12 cells were increased by GEN. The leakage rate of lactic dehydrogenase was decreased in DAI and in GEN-DAI combination treatments, the glutathione contents were increased to (297.27±9.47) μmol/(g·prot) and (269.90±00) μmol/(g·prot) respectively. The acetylcholine content in culture medium and metabolism of cholinergic system was increased in GEN-DAI treatment. GEN-DAI combination treatment can more significantly increase the activity of the antioxidant system of PC12 cells than GEN and DAI treatments.

Key words: food processing technology, PC12 cells, soy isoflavones, antioxidant, neuroprotective effect, genistein, daidzein

中图分类号: 

  • TS201
[1] Xu M L, Liu J, Zhu C, et al. Interactions between soy isoflavones and other bioactive compounds: a review of their potentially beneficial health effects[J]. Phytochem Rev, 2015, 14(3):459-467.
[2] Murphy P A, Barua K, Hauck C C. Solvent extraction selection in the determination of isoflavones in soy foods[J]. Journal of Chromatography B, 2002, 777(1/2):129-138.
[3] Vacek J, Klejdus B, Lojkova L, et al. Current trends in isolation, separation, determination and identification of isoflavones: a review[J]. J Sep Sci, 2008, 31(11):2054-2067.
[4] Donzelli A, Braida D, Finardi A, et al. Neuroprotective effects of genistein in Mongolian gerbils: estrogen receptor-β involvement[J]. J Pharmacol Sci, 2010, 114(2):158-167.
[5] Valles S L, Dolz-Gaiton P, Gambini J, et al. Estradiol or genistein prevent Alzheimer's disease-associated inflammation correlating with an increase PPARc expression in cultured astrocytes[J]. Brain Res, 2010, 1312:138-144.
[6] Kim D K, Chancellor M B. Is estrogen for urinary incontinence good or bad?[J].Rev Urol, 2006,8(2):91-92.
[7] Allred C D, Allred K F, Ju Y H, et al. Soy processing influences growth of estrogen-dependent breast cancer tumors[J]. Carcinogenesis, 2004, 25(9):1649-1657.
[8] Goldstein D S, Sullivan P, Cooney A, et al. Vesicular uptake blockade generates the toxic dopamine metabolite 3, 4-dihydroxyphenylacetaldehyde in PC12 cells: relevance to the pathogenesis of Parkinson's disease[J]. J Neurochem, 2012, 123(6):932-943.
[9] Liu J, Chen Z, He J, et al. Anti-oxidative and anti-apoptosis effects of egg white peptide, Trp-Asn-Trp-Ala-Asp, against H 2 O 2 -induced oxidative stress in human embryonic kidney 293 cells[J]. Food Funct, 2014, 5(12):3179-3188.
[10] 陈志飞. 蛋清源抗氧化肽对HEK293细胞氧化应激损伤的抑制作用及机制研究[D]. 长春:吉林大学军需科技学院, 2014.
Chen Zhi-fei. The aiti-oxidative effects and mecanism of antioxidant peptides from egg hite against oxidantive stress injury in human embryonic kidney 293 cells[D]. Changchun: College of Quartermaster Technology, Jilin University, 2014.
[11] 赵凡桂, 王文君, 周文江, 等. 更年春方对切除卵巢大鼠学习记忆能力和海马胆碱能系统的影响[J]. 中国中西医结合杂志, 2008, 28(3):234-237.
Zhao Fan-gui, Wang Wen-jun, Zhou Wen-jiang, et al. Effect of gengianchun recipe on learning memory function and hippocampal cholinergic system in ovariectomized rats[J]. Chinese Journal of Integrated Traditional and Western Medicine, 2008, 28(3):234-237.
[12] 蔡标, 汪远金, 王艳, 等. 大豆异黄酮对阿尔茨海默病大鼠乙酰胆碱代谢的影响[J]. 安徽中医药大学学报, 2013, 32(1):57-60.
Cai Biao, Wang Yuan-jin, Wang Yan,et al. Effect of soybean isoflavone on acetylcholine metabolism in rats with Alzheimer's disease[J]. Journal of Anhui University of Chinese Medicine, 2013, 32(1):57-60.
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