›› 2012, Vol. ›› Issue (06): 1608-1612.

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Engineering bacteria construction of ginseng β-AS gene RNA interference plant expression vector

ZHAO Shou-jing, CAO Hao-jie, HE Mu-yang, WANG Le   

  1. College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, China
  • Received:2011-12-29 Online:2012-11-01

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Abstract: In this study, the ginseng β-AS gene RNA interference (RNAi) plant expression vector is built through the Recombinant PCR reaction and restriction enzyme digestion connection. Then,by heating, the β-AS gene RNAi is transformed into the Agrobacterium A4 to get β-AS RNAi plant expression vector engineered bacteria. This lays the foundation for further induced transformation of ginseng explants.

Key words: bioengineering, ginsenoside, β-AS, RNAi plant expression vector, engineering bacteria

CLC Number: 

  • Q81
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