The effect of hyperon isospin interaction on the transition density of hyperon stars was studied by means of relativistic mean field theory for the baryon octet {n,p,Λ,Σ-,Σ0,Σ+,Ξ-,Ξ0} system. The results show that, considering the hyperon isospin interaction, the transition density of hyperon stars gradually decreased when xρΣ=2,1,2/3. When baryonic density was the transition density of hyperon stars, the Λ and Ξ- hyperons make the most contribution to the transition density of hyperon stars, Λ and Ξ- hyperon populations nearly amount to 80% of the total hyperon populations, the particle populations of Ξ- decreased as xρΣ reduced, Λ hyperon populations were 34.2%,35.8% and 33.8% of the total hyperon populations as xρΣ=2,1,2/3, respectively.