黄芩素对大鼠神经病理痛的镇痛作用及其机制

doi:10.13481/j.1671-587X.20220307

吉林大学学报(医学版) ›› 2022, Vol. 48 ›› Issue (3): 600-605.doi: 10.13481/j.1671-587X.20220307

• 基础研究 • 上一篇

黄芩素对大鼠神经病理痛的镇痛作用及其机制

刘晓华¹(),房冰莹¹,韩曼¹,乔海法²,于远望¹

^1.陕西中医药大学生理学教研室, 陕西咸阳 712046
^2.陕西省针药结合重点实验室, 陕西咸阳 712046

收稿日期:2021-08-30 出版日期:2022-05-28 发布日期:2022-06-21
通讯作者: 刘晓华 E-mail:xiaohualiu80@163.com
作者简介:刘晓华（1980－），女，陕西省旬邑县人，教授，医学博士，主要从事神经生物学方面的研究。
基金资助:
国家自然科学基金项目(82004221);陕西省科技厅自然科学基金项目(2021JM-477);陕西中医药大学学科创新团队建设项目(2019-YS06)

Analgesic effect of baicalein on neuropathic pain of rats and its mechanism

Xiaohua LIU¹(),Bingying FANG¹,Man HAN¹,Haifa QIAO²,Yuanwang YU¹

^1.Department of Physiology, Shaanxi University of Chinese Medicine, Xianyang 712046, China
^2.Key Laboratory of Acupuncture and Drug Combination, Shaanxi Province, Xianyang 712046, China

Received:2021-08-30 Online:2022-05-28 Published:2022-06-21
Contact: Xiaohua LIU E-mail:xiaohualiu80@163.com

摘要/Abstract

摘要： 目的

观察黄芩素对大鼠坐骨神经慢性压迫引起痛行为的抑制作用及初级感觉神经元中胶质细胞源性神经营养因子（GDNF）表达的调控作用，阐明黄芩素对神经病理痛的镇痛机制。

方法

48只健康雄性SD大鼠随机分为假手术组、模型组和黄芩素组，每组16只。各组大鼠麻醉后，假手术组大鼠仅暴露坐骨神经，模型组和黄芩素组大鼠行坐骨神经慢性缩窄手术建立大鼠慢性缩窄性神经损伤（CCI）模型。造模成功后黄芩素组大鼠腹腔连续注射黄芩素7 d。手术前后不同时间点分别检测各组大鼠患侧机械缩足反射阈值（MWT）和热缩足反射潜伏期（TWL）以评价神经损伤引起的痛行为。第11天，每组选取6只大鼠，免疫荧光染色检测各组大鼠脊髓背角组织中c-Fos阳性细胞数；第28天检测各组其余大鼠背根神经节（DRG）中GDNF荧光强度。

结果

手术前各组大鼠MWT和TWL比较差异无统计学意义（P>0.05）；与假手术组比较，术后1 d模型组和黄芩素组大鼠MWT 和TWL 明显降低（P<0.05），给药后3 d黄芩素组大鼠MWT和TWL升高（P>0.05）；与假手术组比较，模型组大鼠脊髓背角组织中c-Fos阳性细胞数升高（P<0.05）；与模型组比较，黄芩素组大鼠脊髓背角组织中c-Fos阳性细胞数降低（P<0.05）。与假手术组比较，模型组大鼠DRG中GDNF荧光强度降低（P<0.05）；与模型组比较，黄芩素组大鼠DRG中GDNF荧光强度升高（P<0.05）。

结论

黄芩素对大鼠神经病理痛具有镇痛作用。初级感觉神经元中GDNF参与坐骨神经损伤引起的神经病理痛，黄芩素对神经病理痛的镇痛作用可能与其调控GDNF的表达有关。

关键词: 黄芩素, 神经病理痛, 胶质细胞源性神经营养因子, 机械缩足反射阈值, 热缩足反射潜伏期

Abstract: Objective

To observe the inhibitory effect of baicalein on the pain behavior caused by chronic sciatic nerve compression in the rats and the regulation effect of glial cell-derived neurotrophic factor （GDNF） expression in the primary sensory neurons， and to clarify the analgesic mechanism of baicalein in the neuropathic pain.

Methods

A total of 48 healthy male SD rats were randomly divided into sham operation group， model group and baicalein group，and there were 16 rats in each group. After anesthesia，the sciatic nerve of the rats in sham operation group was exposed， and the chronic constricting nerve injury of the rats in model group and baicalein group was performed to establish the chronic constriction injury（CCI） models. The rats in baicalein group were injected with baicalein continuously for 7 d after successful modeling. The mechanical withdraw threshold （MWT） and thermal withdrawal latency （TWL） of the rats in various groups were measured at different time points before and after operation to evaluate the pain behavior caused by nerve injury. On the 11th day，6 rats were randomly selected from each group for immunofluorescence staining to determine the number of C-Fos positive neurons in spinal dorsal horn tissue of the rats in various groups. On the 28th day， the fluorescence intensities of GDNF in dosal root ganglion（DRG） of the rats in various groups were detected.

Results

There was no significant difference between MWT and TWL of the rats in various groups before operation （P>0.05）. Compared with sham operation group， the MWT and TWL of the rats in model group and baicalein group were decreased significantly 1 d after operation （P<0.05）， and they were increased in baicalein group 3 d after administration （P>0.05）. Compared with sham operation group， the number of c-Fos positive neurons in spinal dorsal horn tissue of the rats in model group was increased （P<0.05）； compared with model group， the number of c-Fos positive neurons in spinal dorsal horn tissue of the rats in baicalein group was decreased （P<0.05）. Compared with sham operation group， the fluorescence intensity of GDNF in DRG of the rats in model group was decreased （P<0.05）；compared with model group， and the fluorescence intensity of GDNF in DRG of the rats in baicalein group was increased （P<0.05）.

Conclusion

Baicalein has the analgesic effect on neuropathic pain of the rats.The GDNF in primary sensory neurons is involved in the neuropathic pain caused by sciatic nerve injury. The analgesic effect of baicalein on the neuropathic pain may be related to its regulation of GDNF expression.

Key words: Baicalein, Neuropathic pain, Glial cell line-derived neurotropic factor, Mechenaical withdral threshold, Thermal withdrawal latency

中图分类号:

R741.02

刘晓华,房冰莹,韩曼,乔海法,于远望. 黄芩素对大鼠神经病理痛的镇痛作用及其机制[J]. 吉林大学学报(医学版), 2022, 48(3): 600-605.

Xiaohua LIU,Bingying FANG,Man HAN,Haifa QIAO,Yuanwang YU. Analgesic effect of baicalein on neuropathic pain of rats and its mechanism[J]. Journal of Jilin University(Medicine Edition), 2022, 48(3): 600-605.

图/表 4

参考文献 19

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黄芩素对大鼠神经病理痛的镇痛作用及其机制

Analgesic effect of baicalein on neuropathic pain of rats and its mechanism

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