吉林大学学报(医学版) ›› 2013, Vol. 39 ›› Issue (1): 38-41.doi: 10.7694/jldxyxb20130110

• 基础研究 • 上一篇    下一篇

甘草酸二铵对博莱霉素致肺纤维化大鼠血清iNOS及NO水平的影响及其抗肺纤维化的作用机制

辛维娜１，蒋荣娜２，石 卓３，盛 辉1   

  1. 1．吉林大学第一医院呼吸科，吉林 长春 130021；2．吉林省肿瘤医院ICU科，吉林 长春
     130012；3．吉林大学药学院药理学教研室，吉林 长春 130021
  • 收稿日期:2012-09-04 出版日期:2013-01-28 发布日期:2013-01-30
  • 通讯作者: 盛 辉(Tel：0431-88782443，E-mail:shenghuiy @163.com) E-mail:shenghuiy @163.com
  • 作者简介:辛维娜(1986-)，女，山东省临清市人，在读医学硕士，主要从事呼吸系统疾 病研究。
  • 基金资助:

    吉林省科技厅科技发展计划项目资助课题（20110453）

Effects of diammonium glycyrrhizinate on serum iNOS and NO levels in rats with BLM-induced pulmonary fibrosis and its action mechanism of anti-pulmonary fibrosis

XIN Wei-na1，JIANG Rong-na2，SHI Zhuo3，SHENG Hui1   

  1. 1. Department of Respiratory Medicine，Frist Hospital，Jilin University，Changchun 130021，China；2. Department of ICU，Tumor Hospital of Jilin Province， Changchun 130012，China；3. Department of Pharmacology，School of Pharmacy，Jilin University， Changchun 130021，China
  • Received:2012-09-04 Online:2013-01-28 Published:2013-01-30

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摘要： 目的：探讨甘草酸二铵对博莱霉素（BLM）致肺纤维化的作用，阐明其抗肺纤维化的作用机制。方法：36只Wistar大鼠随机分为正常对照组、博莱霉素所致大鼠肺纤维化组(模型组)及甘草酸二铵组，每组12只。气管内一次性注入博来霉素5 mg/kg复制肺纤维化模型。实验第2天开始，甘草酸二铵组大鼠给以甘草酸二铵75 mg?kg-1?d-1干预，采用腹腔给药，对照组及模型组分别给予生理盐水(10 mL/kg)，连续给药27 d。实验第28天处死所有大鼠，腹主动脉取血，测定血清中诱导型一氧化氮合酶（iNOS）及一氧化氮（NO）的水平。同时取大鼠左肺下叶，行HE染色和Masson三联染色观察肺组织病理学变化。结果：光镜下正常对照组大鼠肺组织大致正常，甘草酸二铵组大鼠肺纤维化轻微，而模型组大鼠肺纤维化改变明显。与正常对照组比较，模型组大鼠血清iNOS、 NO水平明显增高（P<0.01），而甘草酸二铵组无明显变化（P>0.05）；甘草酸二铵组大鼠血清iNOS、NO均低于模型组（P<0.05或P<0.01）。结论：甘草酸二铵可以下调大鼠血清中iNOS及NO的表达，从而减轻肺纤维化。

关键词: 肺纤维化, 博莱霉素, 甘草酸二铵, 诱导型一氧化氮合酶, 一氧化氮

Abstract: Abstract:Objective  To explore the effects of diammonium glycyrrhizinate on bleomycin induced pulmonary fibrosis (PF) and to further clarify the underlying fibrotic mechanisms.Methods 36 Wistar rats were randomly divided into normal control group,BLM-induced pulmonary fibrosis group(model group) andglycyrrhizinate group.The pulmonary fibrosis models of rats were established by injecting   BLM via trachea (5 mg/kg once).From the 2nd day after injection,the rats in glycyrrhizinate group were intraperitoneally injected with glycyrrhizinate(75  mg/kg/d),while the rats in other two groups with normal saline injection instead.On the 28th day,all rats were sacrificed,the blood samples were obstained to measure inducible nitric oxide synthase (iNOS) and nitric oxide (NO) levels.Their left lung lobe tissues were removed and used for pathological section examination.Results  The pulmonary tissue of the rats in  normal control  group was normal，and the pulmonary fibrosis of the rats in glycyrrhizinate group was significantiy lighter,and the pulmonary fibrosis of the rats in model group was severe.Compared with normal control group，the serum iNOS and NO levels of rats in model group were significantly increased（P<0.01）,but the serum iNOS and NO levels of rats in glycyrrhizinate group had no changes（P>0.05）；the serum iNOS and NO levels of the rats in glycyrrhizinate group were lower than　those in model group（P<0.05 or P<0.01）.Conclusion  Diammonium glycyrrhizinate can significantly reduce the serum levels ofiNOS and NO in BLM-induced pulmonary fibrosis rats and  alleviate pulmonary fibrosis.  

Key words:  , pulmonary fibrosis, bieomycin, diammonium glycyrrhizinate, inducible nitric oxide synthase, nitric oxide

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