吉林大学学报(医学版)

• 基础研究 • 上一篇    下一篇

银杏叶提取物对牙龈卟啉单胞菌的体外抑菌作用

姜迎春1,李含薇1,李咏梅1,曲莉2,黄相道1   

  1. 1.北华大学口腔医学院口腔内科教研室,吉林 吉林 132013;2.北华大学公共卫生学院劳动卫生学教研室,吉林 吉林 132013
  • 收稿日期:2013-12-09 出版日期:2014-09-28 发布日期:2014-11-24
  • 通讯作者: 李含薇(Tel:0432-64608113,E-mail:li65575147@163.com) E-mail:li65575147@163.com
  • 作者简介:姜迎春(1970-),男,吉林省延吉市人,主治医师,在读医学硕士,主要从事牙周病学方面的研究。
  • 基金资助:

    吉林省科技厅科技发展计划项目资助课题(20130206036YY);吉林省教育厅“十二五”科学技术研究项目资助课题(吉教科合字[2012]第163号)

Bacteriostatic effect of  extract  of  Ginkgo Ailoba leaves on Porphyromonas gingivalis   in vitro

JIANG Ying-chun1, LI Han-wei1,LI Yong-mei1, QU Li2, HUANG Xiang-dao1   

  1. 1.Department of Oral Medicine,School of Stomatology, North China University, Jilin 132013,China;2. Department of Labor Hygiene,  School of Public Health, North China University,Jilin 132013,China )
  • Received:2013-12-09 Online:2014-09-28 Published:2014-11-24

摘要:

目的:探讨银杏叶提取物对牙龈卟啉单胞菌的体外抑菌活性,为研制开发治疗牙周病的新型纯中药抗菌药物提供药理学依据。方法:本实验分为阴性对照组、亚胺培南对照组和不同浓度及剂型的银杏叶提取物组。采用溶剂提取法制取银杏叶提取物,采用打孔法和试管法在厌氧环境下检测银杏叶提取物对牙龈卟啉单胞菌的体外抑菌活性,并与金黄色葡萄球菌和大肠埃希菌对比。通过观察抑菌环直径和测定最小抑菌浓度(MIC)衡量银杏叶提取物的体外抑菌作用。结果:在抑菌环实验中,银杏叶提取物、银杏叶片和银杏叶软胶囊的原液和1∶4的稀释液作用于牙龈卟啉单胞菌,抑菌环直径随着浓度的降低而减小,银杏叶提取物的最大抑菌直径为16.5 mm,银杏叶片和软胶囊最大抑菌直径分别为15.3和14.5 mm,银杏叶提取物抑菌直径明显大于银杏叶片和银杏叶软胶囊(P <0.05),银杏叶片与银杏叶软胶囊抑菌直径比较差异无统计学意义(P>0.05);大肠埃希菌组和金黄色葡萄球菌组得到同样的结果。MIC测定,银杏叶提取物组内比较,银杏叶提取物浓度高于1.95 mg/L时,试管内均无牙龈卟啉单胞菌生长,而大肠埃希菌和金黄色葡萄球菌仍可以生长,浓度高于6.25和12.5 mg/L时大肠埃希菌和金黄色葡萄球菌不生长,银杏叶提取物对牙龈卟啉单胞菌的抑菌作用优于大肠埃希菌和金黄色葡萄球菌。结论:银杏叶提取物对牙龈卟啉单胞菌具有抑菌作用。

关键词:  , 银杏叶提取物;抑菌作用;牙龈卟啉单胞菌;金黄色葡萄球菌;大肠埃希菌

Abstract:

Abstract:Objective To explore the antibacterial activity of extract of Ginkgo biloba leaves on  Porphyromonas gingivalis in vitro , and to provid  pharmacological reference    for developing a new type of antibacterial drugs in the treatment of periodontal disease. Methods  This experiment was divided into negative control group, imipenem  control group and different concentrations and  forms of extract of Ginkgo biloba leaves groups. Solvent extraction method was used to extract the extract of Ginkgo biloba leaves,punching method and test tube method were performed to detect the antibacterial activity of extract of Ginkgo biloba leaves in anaerobic environment in vitro and compared with Staphylococcus  aureus and E.coli. By observing the antibacterial ring diameter and determination of the minimum bacteriostasis concentration (MIC),  the antibacterial activities of extract of Ginkgo biloba leaves in vitro were measured.Results  In the experiment of bacteriostatic ring,Porphyromonas gingivalis was treated with  extract of Ginkgo biloba leaves, Ginkgo biloba leaf tablet and Ginkgo biloba soft capsule  concentrate and 1∶4 diluent,the bacteriostatic ring diameters were decreased with the decreasing  of the concentration.The maximum bacteriostatic diameter  of Ginkgo biloba extract  was 16.5 mm, and the maximum bacteriostasis diameters   of Ginkgo biloba leaf tablet and soft capsule  were 15.3  and 14.5 mm, respectively; the bacteriostatic diameter of the exact of Ginkgo biloba leaves  was bigger than  those  of Ginkgo biloba leaf tablet and Ginkgo biloba soft capsule (P < 0.05), but there  was no statistical difference of bacteriostatic diameter between Ginkgo biloba leaf tablet and Ginkgo biloba soft capsule  (P> 0.05); E. coli and Staphylococcus  aureus  groups get the same results.   When the  concentration of extract of Ginkgo biloba leaves  was more  than 1.95 mg/L, there was no growth of Porphyromonas gingivalis  but  E. coli and Staphylococcus aureusa still grew; only  the concentrations of exact of Girkgo biloba leaves were  more than 6.25  and 12.5 mg/L, E. coli and Staphylococcus aureus didn’t  grow; the bacteriostatic effect of extract of Ginkgo biloba on Porphromonas gingivalis was better  than  E.coli   and Staphylococcus aureus.Conclusion  Extract of Ginkgo biloba  leaves has antibacterial effect on Porphyromonas gingivalis.

Key words: extract of Ginkgo biloba leaves;antibacterial activity;Porphyromonas gingivalis;Staphylococcus aureus;Escherichia , coli

中图分类号: 

  • R285