吉林大学学报(医学版)

• 基础研究 • 上一篇    下一篇

结直肠癌组织中Kiss-1基因启动子甲基化对其表达
水平的影响及其临床意义

陈志华,林素勇,陈绍勤,戴起宝,韩宏景   

  1. 福建医科大学附属第一医院胃肠外科,福建 福州 350005
  • 收稿日期:2013-11-17 出版日期:2014-09-28 发布日期:2014-11-24
  • 通讯作者: 陈绍勤(Tel:0591-87982082,E-mail:chenshaoqin@medmail.com.cn) E-mail:chenshaoqin@medmail.com.cn
  • 作者简介:陈志华(1986-),男,福建省福州市人,医学硕士,主要从事胃 肠道肿瘤外科基础和临床研究。
  • 基金资助:

    国家临床重点专科建设项目资助课题(2014);
    福建省科技厅自然科学基金资助课题(2013J01291)

Effect of Kiss-1 gene promoter methylation 
on its expression in colorectal carcinoma 
tissue and its clinical significance

CHEN Zhi-hua,LIN Su-yong,CHEN Shao-qin,DAI Qi-bao,HAN Hong-jing   

  1. Department of Gastrointestinal Surgery,First Affiliated Hospital,Fujian Medical University, Fuzhou 350005,China
  • Received:2013-11-17 Online:2014-09-28 Published:2014-11-24

摘要:

目的:研究结直肠癌组织中Kiss-1基因启动子甲基化状态对Kiss-1基因表达的影响,分析其与结直肠癌临床病理特性的关系及临床意义。方法:收集126例结直肠癌组织及其癌旁正常结直肠组织,采用甲基化特异性PCR(MSP)检测Kiss-1基因启动子区甲基化状态,采用实时荧光定量 PCR和Western blotting法检测结直肠癌组织和正常结直肠组织中Kiss-1基因mRNA和蛋白的表达水平。结果:结直肠癌组织中Kiss-1基因启动子甲基化阳性率(83.33%)高于癌旁正常结直肠组织(30.16%)(P<0.05)。结直肠癌组织中Kiss-1基因启动子甲基化阳性组Kiss-1基因mRNA和蛋白表达水平均低于Kiss-1基因启动子甲基化阴性组(P<0.05)。结直肠癌组织中T3+T4组Kiss-1基因启动子甲基化阳性率高于T1+T2组(P<0.05),但Kiss-1基因mRNA和蛋白表达水平低于T1+T2组(P<0.05);
中和低分化组Kiss-1基因启动子甲基化阳性率高于高分化组(P<0.05),Kiss-1基因mRNA表达水平低于高分化组(P<0.05);淋巴结转移组甲基化阳性率高于无淋巴结转移组(P<0.05),但Kiss-1基因mRNA及蛋白表达水平低于无淋巴结转移组(P<0.05);远处转移组Kiss-1基因启
动子甲基化阳性率显著高于无远处转移组(P<0.05),但Kiss-1基因mRNA及蛋白表达水平低于无转移组(P<0.05)。Kiss-1基因启动子甲基化阳性率与患者的性别、年龄、肿瘤部位和大小无明显关联(P>0.05)。结论:结直肠癌组织中Kiss-1基因启动子甲基化与Kiss-1基因表达
水平及结直肠癌的恶性转化特性(特别是转移)有关联,Kiss-1基因甲基化状态有望成为评估结直肠癌转移风险的重要指标。

关键词: 结直肠肿瘤, Kiss-1基因, DNA甲基化, 转移

Abstract:

Objective To research the effect of the Kiss-1 gene promoter methylation on the Kiss-1 gene expression in colorectal carcinoma tissue,and to analyze
 the relationship between the Kiss-1 gene methylation and the clinical pathological features of colorectal carcinoma and its clinical significance.
Methods The Kiss-1 gene promotor region methylation,Kiss-1 gene mRNA and protein expressions  were detected respectively by methylation-specific PCR,Real-time PCR and Western blotting method in 126 cases of colorectal carcinoma tissue and para-cacinoma  normal  colorectal tissue.Results The positive rate of Kiss-1 gene methylation in colorectal carcinoma tissue (83.33%) was significantly higher than that in normal tissue (30.16%) (P<0.05).In the cancer tissue,the expression levels of Kiss-1 gene mRNA and protein in Kiss-1 gene promoter methylation positive group was lower than
 that in Kiss-1 gene promoter methylation negative group (P<0.05).The positive rate of Kiss-1 gene promoter methylation in T3+T4 stage group was higher than that in T1+T2 stage group (P<0.05),but the Kiss-1 gene mRNA and protein expression levels were lower than those in T1+T2 stage group (P<0.05).In the poorly and moderately differentiated group,the positive rate of Kiss-1 gene promoter methylation was higher than that in well differentiated group (P<0.05),but the mRNA expression level of Kiss-1 gene was lower than that  in well differentiated group (P<0.05).In lymph node metastasis
 group,the positive rate of Kiss-1 gene promoter methylation was higher than that in lymph node negative group (P<0.05),but the mRNA and protein expression levels were lower than those in lymph node negative group (P<0.05). And the positive rate of Kiss-1 gene promoter methylation in distant metastasis group was higher than that in non-distant metastasis group (P<0.05),but the mRNA and protein expressions levels  were lower than those in non-distant metastasis group (P<0.05).There were no significant associations between the positive rate of Kiss-1 gene promoter methylation and the  gender,age,tumor location,and tumor diameter of the patients (P>0.05).Conclusion The Kiss-1 gene promoter methylation in colorectal carcinoma  tissue is associated with the Kiss-1 gene expression level and the malignant characteristics of colorectal carcinoma;Kiss-1 gene promoter methylation may be used as a reference indicator for predicting the risk of metastasis  of colorectal carcinoma.

Key words: colorectal neoplasms, Kiss-1 gene, DNA methylation, metastasis

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