不同宫颈病变患者血清和宫颈病变组织中凋亡相关因子的表达及其临床意义

doi:10.13481/j.1671-587x.20150125

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (01): 129-134.doi: 10.13481/j.1671-587x.20150125

不同宫颈病变患者血清和宫颈病变组织中凋亡相关因子的表达及其临床意义

于慧玲¹, 李冬梅², 孙勤暖¹, 马洪喜³

1. 内蒙古医科大学病理学教研室, 内蒙古呼和浩特 010059;
2. 长春大学特殊教育学院针灸推拿系, 吉林长春 130022;
3. 吉林大学第一医院病理科, 吉林长春 130021

收稿日期:2014-05-27 发布日期:2015-01-30
通讯作者: 孙勤暖,副教授,硕士研究生导师(Tel:0471-6637678,E-mail:sunqn118@sina.com);马洪喜,副主任技师(Tel:0431-88782631,E-mail:Mahongxi1969@126.com) E-mail:sunqn118@sina.com;Mahongxi1969@126.com
作者简介:于慧玲(1974-),女,内蒙古巴彦淖尔市人,副教授,在读医学博士,主要从事肿瘤病理学研究。
基金资助:
内蒙古自治区科技厅自然科学基金资助课题(2011MS1142)

Expressions of apoptosis-related factors in serum and cervix tissue of patients with different cervical lesions and their clinical significances

YU Huiling¹, LI Dongmei², SUN Qinnuan¹, MA Hongxi³

1. Department of Pathology, Inner Mongolia Medical University, Hohhot 010059, China;
2. Department of Acupuncture and Manipulation, Special Education College, Changchun University, Changchun 130022, China;
3. Department of Pathology, First Hospital, Jilin University, Changchun 130021, China

Received:2014-05-27 Published:2015-01-30

摘要/Abstract

摘要：

目的: 观察凋亡相关因子在不同宫颈病变患者血清和宫颈病变组织中的表达,探讨其与宫颈鳞状细胞癌(鳞癌)临床病理特征的关系。方法: 选取镜检确诊为不同宫颈病变的患者140例,其中子宫颈鳞癌70例、宫颈原位癌(CINⅢ)50例和宫颈炎20例,均空腹抽取静脉血同时留取镜检组织。采用酶联免疫法、实时荧光定量法和免疫组织化学法分别检测患者血清及不同宫颈病变组织中凋亡相关因子——肿瘤抑制基因(p53)、细胞凋亡信号受体(Fas)、肿瘤坏死因子α(TNF-α)和细胞周期素E (cyclin E)蛋白和mRNA表达,并分析其蛋白表达与宫颈鳞癌临床分期、病理类型和淋巴结转移的关系。结果: 与宫颈炎患者比较,宫颈鳞癌和宫颈原位癌患者血清及宫颈组织中p53、Fas、TNF-α蛋白和mRNA表达水平均明显降低(P<0.01),而cyclin E蛋白和mRNA表达水平明显升高(P<0.01),宫颈鳞癌患者与宫颈原位癌患者比较差异无统计学意义(P>0.05);p53、Fas、TNF-α和cyclin E阳性表达率与宫颈鳞癌临床分期、病理类型和淋巴结转移均有密切关联(P<0.01)。结论: 宫颈鳞癌和宫颈原位癌患者血清和宫颈病变组织中p53、Fas和TNF-α表达水平下调和cyclin E表达水平上调可能与宫颈癌的浸润和转移有关联,可作为宫颈癌早期诊断的标记物,并可为宫颈癌的术后用药和预后判断提供理论依据。

关键词: 宫颈肿瘤, 宫颈原位癌, 宫颈炎, 肿瘤抑制基因, 细胞凋亡信号受体, 肿瘤坏死因子&alpha, 细胞周期素E

Abstract:

Objective To observe the expressions of apoptosis-related factors in serum and cervix tissue of the patients with different cervical lesions, and to explore their relationships with the clinicopathological characteristics of cervical squamous cell carcinoma. Methods 140 patients with different cervical lesions diagnosed with microsopic examination were selected, including 70 cases of cervical squamous carcinoma, 50 cases of cervical carcinoma in situ (CIN Ⅲ) and 20 cases of cervicitis, and fasting venous blood was also collected and the cervical tissue for microscopic examination was obtained.The protein and mRNA expressions of p53, Fas, TNF-α and cyclin E in serum and different cervical tissues were detected by ELISA, real-time fluorescence quantitative method and immunohistochemical method;the relationships between the expressions of p53, Fas, TNF- α and cyclin E proteins and the clinical stages, pathological types and lymph node metastasis of cervical squamous cell carcinoma were analyzed. Results Compared with the patients with cervicitis, the protein and mRNA expression levels of p53, Fas, and TNF-α in serum and cervix tissue in the patients with cervical squamous cell carcinoma and cervical carcinoma in situ were decreased(P<0.01), but the expression levels of cyclin E mRNA and protein were significantly increased(P<0.01), there was no statistical significance between the patients with cervical squamous cell carcinoma and the patients with cervical carcinoma in situ (P>0.05).The positive expression rates of p53, Fas, TNF- α and cyclin E had positive relations with the clinical stages, pathological types and lymph node metastasis of cervical squamous cell carcinoma(P<0.01). Conclusion The down-regulation of the expression levels of p53, Fas and TNF-α and the up-regulation of cyclin E expression level in the serum and cervix tissue of the patients with cervical squamous cell carcinoma and cervical carcinoma in situ may be associated with the tumor invasion and metastasis of cervical carcinoma, and they may be served as the markers for early diagnosis of cervical cancer;the study provides guidance for the postoperative treatment and prognosis judgement of cervical cancer.

Key words: uterine cervical neoplasms, cervical carcinoma in situ, cervicitis, tumor suppressor gene, apoptosis signal receptor, tumor necrosis factor alpha, cyclin E

中图分类号:

R711.32

于慧玲, 李冬梅, 孙勤暖, 马洪喜. 不同宫颈病变患者血清和宫颈病变组织中凋亡相关因子的表达及其临床意义[J]. 吉林大学学报(医学版), 2015, 41(01): 129-134.

YU Huiling, LI Dongmei, SUN Qinnuan, MA Hongxi. Expressions of apoptosis-related factors in serum and cervix tissue of patients with different cervical lesions and their clinical significances[J]. Journal of Jilin University Medicine Edition, 2015, 41(01): 129-134.

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不同宫颈病变患者血清和宫颈病变组织中凋亡相关因子的表达及其临床意义

Expressions of apoptosis-related factors in serum and cervix tissue of patients with different cervical lesions and their clinical significances

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