IL-4-33C/T和IL-6-572C/G基因多态性与慢性阻塞性肺疾病易感性的关联性分析

doi:10.13481/j.1671-587x.20150128

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (01): 145-149.doi: 10.13481/j.1671-587x.20150128

IL-4-33C/T和IL-6-572C/G基因多态性与慢性阻塞性肺疾病易感性的关联性分析

张文静, 井宏宇, 许弘邦, 钱东华

吉林大学第一医院呼吸内科, 吉林长春 130021

收稿日期:2014-10-21 发布日期:2015-01-30
通讯作者: 钱东华,教授,硕士研究生导师(Tel:0431-88782443,E-mail:qiandonghua6016@163.com) E-mail:qiandonghua6016@163.com
作者简介:张文静(1987-),女,山东省聊城市人,医学硕士,主要从事肺部感染性疾病方面的临床研究。
基金资助:
吉林省卫生厅科研基金资助课题(2010Z031);吉林大学创新基金资助课题(医学704053)

Analysis on association between polymorphisms of IL-4-33C/T and IL-6-572C/G and susceptibility of chronic obstructive pulmonary disease

ZHANG Wenjing, JING Hongyu, XU Hongbang, QIAN Donghua

Department of Respiratory Diseases, First Hospital, Jilin University, Changchun 130021, China

Received:2014-10-21 Published:2015-01-30

摘要/Abstract

摘要：

目的: 探讨慢性阻塞性肺疾病(COPD)患者的白细胞介素4(IL-4)启动子区域基因-33C/T(IL-4-33C/T)和白细胞介素6(IL-6)启动子区域基因-572C/G(IL-6-572C/G)的单核苷酸多态性(SNPs)与COPD易感性的关系,为COPD的遗传学机制研究提供理论依据。方法: 采用病例-对照研究方法,选取100例COPD患者作为病例组,选取与病例组患者年龄性别匹配的100名健康人作为对照组,采用PCR-RELP方法检测病例组和对照组受试者的基因型,应用SPSS 19.0统计软件分析SNPs位点与COPD易感性的关联性。结果: 病例组和对照组IL-4-33C/T和IL-6-572C/G位点的基因型频数分布均符合Hardy-Weinberg平衡定律(P>0.05);病例组和对照组IL4 -33C/T位点的基因型和等位基因频数分布比较差异无统计学意义(P>0.05),IL-6-572C/G位点的基因型和等位基因频数分布比较差异有统计学意义(χ²=17.398,P<0.001;χ²=23.169,P<0.001)。结论: IL-4-33C/T位点的基因多态性可能与COPD易感性无关联,而IL-6 -572C/G位点基因多态性与COPD易感性有关联。

关键词: 慢性阻塞性肺疾病, 白细胞介素4, 白细胞介素6, 基因多态性

Abstract:

Objective To investigate the association between the single nucleotide polymorphisms (SNPs) of interleukin 4 (IL-4) promoter region gene-33C/T(IL-4-33C/T), interleukin-6 (IL-6) promoter region gene-572C/G(IL-6-572C/G) and the susceptibility of chronic obstructive pulmonary disease (COPD), and to provide theoretical basis for genetic mechanism of COPD. Methods A case-control study was used in this research.100 cases of COPD were selected as case group, and 100 healthy people matched in age and sex were selected as control group.PCR-RELP analysis was employed to detect the genotypes of the subjects in two groups, and statistical analysis software SPSS19.0 was used to analyze the association between IL-4 -33C/T, IL-6 -572C/G and susceptibility of COPD. Results The distribution of genotypic frequencies of IL-4 -33C/T and IL-6 -572C/G sites didn't deviate from Hardy-Weinberg equilibrium in both case group and control group(P>0.05).The distribution of genotypic and allelic frequencies at IL-4 -33C/T site showed no statistical significance between case group and control group(P>0.05).The distribution of genotypic and allelic frequencies at IL-6 -572C/G site showed statistical significance between case group and control group(χ²=17.398, P<0.001;χ²=23.169, P<0.001). Conclusion The polymorphism of IL-4-33C/T site may be not associated with the susceptibility of COPD.The polymorphism of IL-6-572C/G site may be associated with the susceptibility of COPD.

Key words: chronic obstructive pulmonary disease, interleukin-4, interleukin-6, gene polymorphism

中图分类号:

R563.9

张文静, 井宏宇, 许弘邦, 钱东华. IL-4-33C/T和IL-6-572C/G基因多态性与慢性阻塞性肺疾病易感性的关联性分析[J]. 吉林大学学报(医学版), 2015, 41(01): 145-149.

ZHANG Wenjing, JING Hongyu, XU Hongbang, QIAN Donghua. Analysis on association between polymorphisms of IL-4-33C/T and IL-6-572C/G and susceptibility of chronic obstructive pulmonary disease[J]. Journal of Jilin University Medicine Edition, 2015, 41(01): 145-149.

参考文献

[1] 中华医学会呼吸病学分会慢性阻塞性肺疾病学组.慢性阻塞性肺疾病诊治指南(2013年修订版)[J].中华结核和呼吸杂志,2013,36(4):255-264.

[2] 王伟,余亚娟,黄翠萍,等.IL-13、IL-4和β2-肾上腺素受体基因多态性与慢性阻塞性肺疾病易感性关系的研究[J].临床内科杂志,2011,28(5):332-334.

[3] van Durme YM,Lahousse L,Verhamme KM,et al.Mendelian randomization study of interleukin-6 in chronic obstructive pulmonary disease [J].Respiration,2011,82(6):530-538.

[4] Chung KF.Cytokines in chronic obstructive pulmonary disease [J].Eur Respir J,2001,34(18): 50s-59s.

[5] Venkayya R,Lam M,Willkom M,et al.The Th2 lymphocyte products IL-4 and IL-13 rapidly induce airway hyperresponsiveness through direct effects on resident airway cells[J].Am J Respir Cell Mol Biol,2002,26(2):202-208.

[6] D'amato M,Vitiani LR,Petrelli G,et al.Association of persistent bronchial hyperresponsiveness with β2-adrenoceptor(ADRB2) haplotypes:a population study[J].Am J Respir Crit Care Med,1998,158(6):1968-1973.

[7] 窦丽阳.IL4、IL13及受体基因多态性与中国西南地区汉族人群慢性阻塞性肺疾病易感相关性的研究[D].成都:四川大学,2007.

[8] Trajkov D,Mirkovska-Stojkovikj J,Petlichkovski A,et al.Association of cytokine gene polymorphisms with chronic obstructive pulmonary disease in Macedonians[J].Iran J Allergy Asthma Immunol,2009,8(1): 31-42.

[9] Shukla RK,Kant S,Bhattacharya S,et al.Association of cytokine gene polymorphisms in patients with chronic obstructive pulmonary disease[J].Oman Med J,2012,27(4): 285-290.

[10] van der Pouw-Kraan TC,Kucukaycan M,Bakker AM,et al.Chronic obstructive pulmonary disease is associated with the-1055 IL-13 promoter polymorphism[J].Genes Immun,2002,3(7):436-439.

[11] Brull DJ, Montgomery HE,Sanders J, et al.Interleukin-6 gene -174G>C and -572G>C promoter polymorphisms are strong predictors of plasma interleukin-6 levels after coronary artery bypass surgery [J].Arterioscler Thromb Vasc Biol,2001,21(9):1458-1463.

[12] Hsieh MH,Chong IW,HwangJJ,et al.Lack of associations between several polymorphisms in cytokine genes and the risk of chronic obstructive pulmonary diseases in Taiwan[J].Kaohsiung J Med Sci,2008,24(3):126-137.

[13] Cordoba-Lanus E,de-Torres JP,Lopez-Aguilar C,et al.Association of IL-6 gene polymorphisms and COPD in a Spanish population[J].Respir Med,2008,102(12):1805-1811.

[14] Eddahibi S,Chaouat A,TuL,et al.Interleukin-6 gene polymorphism confers susceptibility to pulmonary hypertension in chronic obstructive pulmonary disease[J].Proc Am Thorac Soc,2006,3(6): 475-476.

[15] 陆晶晶,梁永杰,朱辉,等.白细胞介素-6基因多态性与慢性阻塞性肺疾病及吸烟因素的相关性研究[J].临床内科杂志,2013,30(10):669-672.

[16] 周爱莲.IL-6 基因启动子多态性与 COPD 相关性分析[J]. 中华全科医学, 2012,10(6):1257-1258.

IL-4-33C/T和IL-6-572C/G基因多态性与慢性阻塞性肺疾病易感性的关联性分析

Analysis on association between polymorphisms of IL-4-33C/T and IL-6-572C/G and susceptibility of chronic obstructive pulmonary disease

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