DNMT3b基因多态性及其在胃癌组织中的表达水平与胃癌的关联性分析

doi:10.13481/j.1671-587x.20150232

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (02): 369-373.doi: 10.13481/j.1671-587x.20150232

DNMT3b基因多态性及其在胃癌组织中的表达水平与胃癌的关联性分析

王川¹, 贾志芳¹, 曹东慧¹, 武兴¹, 曹雪源², 姜晶¹

1. 吉林大学第一医院临床流行病学研究中心, 吉林长春 130021;
2. 吉林大学第一医院胃结直肠外科, 吉林长春 130021

收稿日期:2014-07-08 出版日期:2015-03-28 发布日期:2015-04-04
通讯作者: 曹雪源, 副教授, 硕士研究生导师(Tel:0431-81875609, E-mail:caoxy@aliyun.com.cn);姜晶, 副教授, 硕士研究生导师(Tel:0431-81875408, E-mail:jiangjing19702000@jlu.edu.cn) E-mail:caoxy@aliyun.com.cn;jiangjing19702000@jlu.edu.cn
作者简介:王川(1989-), 女, 河北省张家口市人, 在读医学硕士, 主要从事胃肠道肿瘤的流行病学方面的研究。
基金资助:
国家自然科学基金资助课题(81072369,81273065,81373084)

Associations between polymorphisms of DNMT3b gene and itsexpression level in gastric cancer tissue and gastric cancer

WANG Chuan¹, JIA Zhifang¹, CAO Donghui¹, WU Xing¹, CAO Xueyuan², JIANG Jing¹

1. Department of Clinical Epidemiology, First Hospital, Jilin University, Changchun 130021, China;
2. Department of Gastric and Colorectal Surgery, First Hospital, Jilin University, Changchun 130021, China

Received:2014-07-08 Online:2015-03-28 Published:2015-04-04

摘要/Abstract

摘要：

目的:探讨中国北方汉族人群DNA甲基转移酶3b(DNMT3b)基因单核苷酸多态性(SNPs)和胃癌发病风险之间的关联性,阐明DNMT3b基因是否为胃癌的易感基因。方法:选择447例胃癌患者作为病例组,961名健康对照者作为对照组,采用TaqMan技术检测2组研究对象DNMT3b基因的rs1569686、rs6119954、rs4911107、rs4911259和rs8118663位点的基因型分布。免疫组织化学技术检测病例组(104例)胃癌患者胃癌组织和85例患者的癌旁对照组织中DNMT3b蛋白的表达水平。结果:在病例组和对照组研究对象中5个SNPs位点基因型分布频率相近,未发现5个SNPs位点的多态性与胃癌的发生有关联(P>0.05)。与癌旁组织比较,胃癌组织中DNMT3b蛋白的表达水平升高(P<0.05)。5个SNPs的不同基因型与胃癌组织中DNMT3b蛋白的表达水平均无关联(P>0.05)。结论:中国北方汉族人群中DNMT3b基因多态性与胃癌的发生无关联,DNMT3b基因可能不是胃癌的易感基因。

关键词: DNA甲基转移酶3b, 胃肿瘤, 单核苷酸多态性

Abstract:

Objective To investigate the associations between single nucleotide polymorphisms(SNPs)of DNA methyltransferase 3b(DNMT3b) gene and the risk of gastric cancer in the Han population from North China,and to clarify whether DNMT3b gene is the susceptible gene of gastric cancer.Methods 447 patients with gastric cancer were selected as case group and 961 healthy controls were used as control group.The distribution of genotypic frequencies of five SNPs(rs6119954,rs1569686,rs4911107,rs4911259,and rs8118663)in two groups were detected by TaqMan assay.The expression levels of DNMT3b protein in 104 cases of cancer tissue from the patients with gastric cancer and 85 cases of tumor-adjacent tissue from the patients with gastric cancer were analyzed by immunohistochemistry method.Results The distributions of genotypic frequencies of five SNPs in case group and control group were similar.No significant association was found between the five SNPs and the risk of gastric cancer(P>0.05).Compared with the tumor-adjacent tissue,the expression level of DNMT3b protein in the gastric cancer tissue was increased(P<0.05).There were no siginificant differences between the genotypes of the five SNPs and the expression levels of DNMT3b protein(P>0.05).Conclusion There is no association between the polymorphisms of DNMT3b and the occurrence of gastric cancer in Han population from North China.DNMT3b gene may not be the susceptible gene of gastric cancer.

Key words: DNA methyltransferase 3b, stomach neoplasms, sigle nucleotide polymorphisms

中图分类号:

R735.2

王川, 贾志芳, 曹东慧, 武兴, 曹雪源, 姜晶. DNMT3b基因多态性及其在胃癌组织中的表达水平与胃癌的关联性分析[J]. 吉林大学学报(医学版), 2015, 41(02): 369-373.

WANG Chuan, JIA Zhifang, CAO Donghui, WU Xing, CAO Xueyuan, JIANG Jing. Associations between polymorphisms of DNMT3b gene and itsexpression level in gastric cancer tissue and gastric cancer[J]. Journal of Jilin University Medicine Edition, 2015, 41(02): 369-373.

参考文献

[1] Ferlay J,Shin HR,Bray F,et al. Estimates of worldwide burden of cancer in 2008:GLOBOCAN 2008 [J].Int J Cancer,2010,127(12):2893-2917.

[2] Saeki N,Ono H,Sakamoto H,et al.Genetic factors related to gastric cancer susceptibility identified using a genome-wide association study [J].Cancer Sci,2013,104(1):1-8.

[3] Jeltsch A.Molecular enzymology of mammalian DNA methyltransferases [J].Curr Top Microbiol Immunol,2006,301:203-225.

[4] 张丽丽,吴建新.DNA甲基化——肿瘤产生的一种表观遗传学机制 [J].遗传,2006, 28(7):880-885.

[5] 殷科,盛贤能,郭宇,等.DNMT1、DNMT3b在胃癌中的表达及其临床意义 [J].现代实用医学,2013,25(12):1386-1388.

[6] Daraei A,Salehi R,Mohamadhashem F.DNA-methyltransferase 3B 39179 G > T polymorphism and risk of sporadic colorectal cancer in a subset of Iranian population [J].J Res Med Sci,2011,16(6):807-813.

[7] Lee SJ,Jeon HS,Jang JS,et al.DNMT3B polymorphisms and risk of primary lung cancer [J].Carcinogenesis,2005,26(2):403-409.

[8] Samudio-Ruiz SL,Hudson LG.Increased DNA methyltransferase activity and DNA methylation following epidermal growth factor stimulation in ovarian cancer cells [J].Epigenetics,2012,7(3):216-224.

[9] 鲍倩,何帮顺,陈丽萍,等.DNA甲基转移酶3B基因启动子单核苷酸多态性与结直肠癌的相关性 [J].中华预防医学杂志,2012,46(1):53-57.

[10] Wang YM,Wang R,Wen DG,et al.Single nucleotide polymorphism in DNA methyltransferase 3B promoter and its association with gastric cardiac adenocarcinoma in North China [J].World J Gastroenterol,2005,11(23):3623-3627.

[11] Hu JB,Fan H,Liu DS,et al.DNMT3B promoter polymorphism and risk of gastric cancer [J].Dig Dis Sci,2010,55(4):1011-1016.

[12] Shen HB,Wang L,Spitz MR,et al.A novel polymorphism in human cytosine DNA-methyltransferase-3B promoter is associated with an increased risk of lung cancer [J].Cancer Res,2002,62(17):4992-4995.

[13] Montgomery KG,Liu MC,Eccles DM,et al.The DNMT3B C→T promoter polymorphism and risk of breast cancer in a British population:a case-control study [J].Breast Cancer Res, 2004,6(4):390-394.

[14] Yang XX,He XQ,Li FX,et al.Risk-association of DNA methyltransferases polymorphisms with gastric cancer in the Southern Chinese population [J].Int J Mol Sci,2012,13(7):8364-8378.

[15] Aung PP,Matsumura S,Kuraoka K,et al.No evidence of correlation between the single nucleotide polymorphism of DNMT3B promoter and gastric cancer risk in a Japanese population [J].Oncol Rep,2005,14(5):1151-1154.

[16] Su XW,Lv CY,Qiao FC,et al.Expression pattern and clinical significance of DNA methyltransferase 3B variants in gastric carcinoma [J].Oncol Rep,2010,23(3):819-826.

[17] 周宁,李象霖,张月萍,等.甘肃地区人群雌激素受体α-29位基因多态性与HBV感染转归的关系[J].临床肝胆病杂志,2014,30(2):128-131.

DNMT3b基因多态性及其在胃癌组织中的表达水平与胃癌的关联性分析

Associations between polymorphisms of DNMT3b gene and itsexpression level in gastric cancer tissue and gastric cancer

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