吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (05): 919-924.doi: 10.13481/j.1671-587x.20150507

• 基础研究 • 上一篇    下一篇

奥曲肽对谷氨酸钠诱发的Aδ和C纤维放电活性的抑制效应

罗蓉1, 欧阳征鹏2, 郭媛3, 姚繁荣3, 李莉3, 王军3, 马少杰3, 赵晏3   

  1. 1. 成都医学院体温与炎症四川省高校重点实验室, 四川 成都 610083;
    2. 四川省医学科学院四川省人民医院 心血管超声及心功能科, 四川 成都 610072;
    3. 西安交通大学医学院生理学与病理生理学系, 陕西 西安 710061
  • 收稿日期:2014-12-11 出版日期:2015-09-28 发布日期:2015-09-29
  • 通讯作者: 赵晏,教授,博士研究生导师(Tel:029-82655171,E-mail:zhaoy502@mail.xjtu.edu.cn) E-mail:zhaoy502@mail.xjtu.edu.cn
  • 作者简介:罗蓉(1977-),女,湖北省荆州市人,副研究员,理学博士,主要从事外周痛觉信息传递的分子学机制方面的研究。
  • 基金资助:

    国家自然科学基金资助课题(81160141,81200604)

Inhibitory effect of octreotide on sodium glutamate-evoked activation of Aδ and C fibers

LUO Rong1, OUYANG Zhengpeng2, GUO Yuan3, YAO Fanrong3, LI Li3, WANG Jun3, MA Shaojie3, ZHAO Yan3   

  1. 1. Key Laboratory of Thermoregulation and Inflammation of Sichuan Higher Education Institutes, Chengdu Medical College, Chengdu 610083, China;
    2. Department of Cardiovascular Ultrasonics and Cardiac function, Sichuan Academy of Medical Sciences, Sichuan Provincial People's Hospital, Chengdu 610072, China;
    3. Department of Physiology and Pathophysiology, School of Medical Sciences, Xi'an Jiaotong University, Xi'an 710061, China
  • Received:2014-12-11 Online:2015-09-28 Published:2015-09-29

摘要:

目的:探讨生长抑素激动剂奥曲肽是否通过抑制兴奋性神经递质谷氨酸的作用而产生抗伤害感受作用。方法:在DA大鼠脊神经背侧皮支分离细束,随机分为对照组、谷氨酸钠组、奥曲肽组和环生长抑素组。观察大鼠感受野注射谷氨酸钠、奥曲肽或环生长抑素预处理后Aδ和C纤维放电率。结果:与对照组比较,谷氨酸钠组大鼠Aδ 及C纤维放电率明显升高(P<0.05);与谷氨酸钠组比较,奥曲肽组大鼠注射过程中以及注射后5 min内Aδ和C纤维放电率明显降低(P<0.05);与谷氨酸钠组比较,环生长抑素组大鼠注射过程中以及注射后5 min内平均放电率差异无统计学意义(P>0.05)。结论:外周生长抑素受体激动剂奥曲肽通过抑制谷氨酸钠诱导的外周Aδ 和C纤维放电活性而抑制伤害感受器。

关键词: 奥曲肽, 谷氨酸钠, A&delta, 纤维, C纤维, 电活性

Abstract:

Objective To investigate whether octreotide,a somatostatin receptor agonist,plays antinociceptive effect by inhibiting the role of exciting neurotransmitter glutamate in periphery. Methods The nerve filaments on the dorsal cutaneous branches of spinal nerve of DA rats were dissected.The filaments were randomly divided into control group,sodium glutamate group,octreotide group,and cyclo-somatostatin group.The discharge rate of Aδ and C fibers were observed after injected with sodium glutamata,octreotide,and cyclo-somatos tatin into the receptive field of rats. Results Compare with control group,the discharge rate of Aδ and C fibers were increased significantly in sodium glutamate group(P<0.05).The discharge rate of Aδ and C fibers of the rats in octreotide group were significantly decreased than those in sodium glutamate group during injection and 5 min after injection(P<0.05);however,there were no significant differences in the discharge rates of Aδ and C fibers of the rats between cyclo-somatostatin group and sodium glutamate group during injection and 5 min after injection(P<0.05). Conclusion Octreotide,a somatostatin receptor agonist,inhibits the nociceptor by reducing glutamate-evoked activation of Aδ and C fibers.

Key words: octreotide, sodium glutamate, A&delta, fibers, C fibers, activation

中图分类号: 

  • R338.8