局部注射熊果酸对大鼠正畸牙移动和牙根吸收的减缓作用及其机制

doi:10.13481/j.1671-587x.20160208

摘要/Abstract

摘要：

目的: 探讨局部注射熊果酸对大鼠正畸牙移动距离和牙根吸收的减缓作用,初步阐明其作用机制。方法: 选取96只雄性Wistar大鼠建立正畸牙移动模型,将建模成功后的大鼠随机分为0(对照组)、0.5、1.0和2.0 mmol·L^-1熊果酸组,每组24只。将不同剂量熊果酸局部注射于各组大鼠右侧上颌第1磨牙近中颊侧黏骨膜下,每3d 1次,每次50 μL。分别于加力1、3、5、7、10、14、21和28d后测量各组大鼠右侧上颌第1磨牙移动距离,采用HE染色观察各组大鼠牙根组织的形态学变化。结果: 对照组、0.5、1.0和2.0 mmol·L^-1熊果酸组大鼠牙移动距离均随时间延长而增加(P<0.01)。与对照组比较,0.5 mmol·L^-1熊果酸组在加力3、7、14、21和28d后大鼠牙齿移动距离明显减小(P<0.05或P<0.01);1.0和2.0 mmol·L^-1熊果酸组在加力1d后大鼠牙齿移动距离差异无统计学意义(P>0.05),其他时间点的移动距离比较差异均有统计学意义(P<0.01)。0.5、1.0和2.0 mmol·L^-1熊果酸组组间两两比较,在加力5、7、10、14、21和28d后大鼠牙齿移动距离差异均有统计学意义(P<0.05或P<0.01)。大鼠牙根组织形态学,随加力时间延长,牙根表面开始出现骨吸收陷窝;随着熊果酸剂量的增加,牙根表面的吸收情况有所缓解。结论: 局部注射0.5、1.0和2.0 mmol·L^-1熊果酸可减小大鼠正畸牙移动的距离,随剂量增加,大鼠牙齿移动距离减小,且熊果酸有减缓正畸所致牙根吸收的作用。

关键词: 熊果酸, 正畸牙移动, 牙根吸收, 大鼠, Wistar

Abstract:

Objective: To investgate the ease effects of local injection of ursolic acid both on orthodontic tooth movement distance and tooth root resorption in the rats, and to clarify its mechanism.Methods: Ninety-six male Wistar rats were used to establish models of orthodontic tooth movement. After the successful establishment of models, all the model rats were randomly divided into 0 (control group), 0.5, 1.0 and 2.0 mmol·L^-1 ursolic acid groups(n=24).The rats were locally injected with different doses of ursolic acid into the palatal submucosal area adjacent to the right upper first molar, once per 3 d, each time 50 μL.The rats were respectively sacrificed on the days 1, 3, 5, 7, 10, 14, 21 and 28, then the distances of tooth movement were measured.HE staining was used to observe the morphologic changes of the root tissue of rats.Results: The tooth movement distances of rats in control, 0.5, 1.0, and 2.0 mmol·L^-1 ursolic acid groups were increased with the prolongation of time(P<0.01).Compared with control group, the tooth movement distances of rats in 0.5 mmol·L^-1 ursolic acid group 3, 7, 14, 21 and 28 d after forcing had statistical significance(P<0.05 or P<0.01); the tooth movement distances of rats in 1.0 and 2.0 mmol·L^-1 ursolic acid groups 1 d after treatment had no statistical significance(P>0.05), but at the other time points, the tooth movement distances of rats had statistical significance(P<0.01). The tooth movement distances of rats had statistical significance between 0.5, 1.0 and 2.0 mmol·L^-1 ursolic acid groups 5, 7, 10, 14, 21 and 28 d after forcing (P<0.05 or P<0.01). The morphological results showed that the resorption pits were found on the surface of tooth root with the prolongation of loading time;in addition, with the increasing of the doses of ursolic acid, the absorption of root surface was relieved. Conclusion: Local injection of ursolic acid at the doses of 0.5, 1.0 and 2.0 mmol·L^-1 can reduce the distance of orthodontic tooth movement in the rats, and the distance of tooth movement in the rats is reduced with the increasing of its doses, and ursolic acid has the ease effect on the orthodoutic tooth root resorption.

Key words: ursolic acid, orthodontic tooth movement, root resorption, rats,Wistar

中图分类号:

R783.5

崔跃, 姜欢, 崔聪聪, 胡敏. 局部注射熊果酸对大鼠正畸牙移动和牙根吸收的减缓作用及其机制[J]. 吉林大学学报(医学版), 2016, 42(02): 231-235.

CUI Yue, JIANG Huan, CUI Congcong, HU Min. Ease effects of local injection of ursolic acid on orthodontic tooth movement and root resorption of rats and their mechanisms[J]. Journal of Jilin University Medicine Edition, 2016, 42(02): 231-235.

参考文献

[1] Roscoe MG, Meira JB, Cattaneo PM. Association of orthodontic force system and root resorption:A systematic review[J].Am J Orthod Dentofac Orthop,2015,147(5):610-626.

[2] Oshiro T, Shibasaki Y, Martin TJ, et al. Immunolocalization of vacuolar type H⁺-ATPase, cathepsin K, matrix metalloproteinase-9,and receptor activator of NF kappaB ligand in odontoclasts during physiological root resorption of human deciduous teeth[J]. Anat Rec,2001, 264(3):305-311.

[3] Hendriks WJ, Elson A, Harroeh S, et al. Protein tyrosine phosphatases:functional inferences from mouse models and human diseases[J]. FEBS J, 2008, 275(5):816-830.

[4] Sheng MH, Wergedal JE, Mohan S, et al. Osteoaetivin is a novel osteoclastic protein and plays a key role in osteoclast differentiation and activity[J]. FEBS Lett, 2008, 582(10):1451-1458.

[5] Jiang H, Sui Y, Cui Y, et al. Expression, purification, and characterization of human osteoclastic protein-tyrosine phosphatase catalytic domain in Escherichia coli[J]. Protein Expr Purif, 2015, 107:7-12.

[6] Amoui M, Suhr SM, Baylink DJ,et al. An osteoclastic proteintyrosine phosphatase may play a role in the differentiation and activity of human monocytic U-937 cell-derived, osteoclast like cells[J]. Am J Physiol Cell Physiol,2004,287(4):C874-C884.

[7] Yang JH, Amoui M, Lau KH. Targeted deletion of the osteoclast protein-tyrosine phosphatase (PTP-oc) promoter prevents RANKL-mediated osteoclastic differentiation of RAW264.7 cells[J].FEBS Lett,2007, 581(13):2503-2508.

[8] 姜欢.PTP-oc抑制剂——熊果酸对破骨细胞分化及正畸牙根吸收的影响[D].长春:吉林大学,2015.

[9] Tan H, Ashour A, Katakura Y, et al. A structure-activity relationship study on anti osteoclastogenesis effect of triterpenoids from the leaves of loquat (Eriobotrya japonica)[J]. Phytomedicine, 2015,22(4):498-503.

[10] Jiang C, Xiao F, Gu X, et al.Inhibitory effects of ursolic acid on osteoclastogenesis and titanium particle induced osteolysis are mediated primarily via suppression of NF-κB signaling[J]. Biochimie, 2015, 111:107-118.

[11] 周艳妮,曹宝成,蒋小龙,等.淫羊藿苷可抑制正畸致牙根的吸收[J].中国组织工程研究,2013,17(28):5171-5176.

[12] Diercke K, Kohl A, Lux CJ, et al. Compression of human primary cementoblasts leads to apoptosis:A possible cause of dental root resorption[J]. J Orofac Orthop, 2014, 75(6):430-445.

[13] Nakano T, Hotokezaka H, Hashimoto M, et al. Effects of different types of tooth movement and force magnitudes on the amount of tooth movement and root resorption in rats[J]. Angle Orthod, 2014, 84(6):1079-1085.

[14] Sawicka M, Bedini R, Wierzbicki PM, et al. Interruptedorthodonticforce results in lessroot resorptionthan continuous force in human premolars as measured by micro-computed tomography[J]. Folia Histochem Cytobiol, 2014, 52(4):289-296.

[15] Jiang RP, McDonald JP, Fu MK. Root resorption before and after orthodontic treatment:a clinical study of contributory factors[J]. Eur J Orthod, 2010, 32(6):693-697.

[16] Oenning AC, Neves FS, Alencar PN, et al. External root resorption of the second molar associated with third molar impaction:comparison of panoramic radiography and cone beam computed tomography[J]. J Oral Maxillofac Surg, 2014, 72(8):1444-1455.

[17] Harris EF, Kineret SE, Tolley EA. A heritable component for external apical root resorption in patients treated orthodontically[J].Am J Orthod Dentofacial Orthop,1997, 111(3):301-309.

[18] 余丽霞,何姝姝,陈嵩.全景及根尖片对正畸相关牙根吸收诊断准确性的研究[J].华西口腔医学杂志, 2012,30(2):169-172.

[19] Brezniak N, Wasscrstein A. Orthodontically induced inflammatory root resorption[J]. Angle Orthod, 2002, 72:175-184.

[20] Krishnan V, Davidovitch Z. Cellular, molecular, and tissue-level reactions to orthodontic force[J]. Am J Orthod Dentofac Orthop, 2006, 129(4):469.e1-469.e32.

[21] Yamaguchi M. RANK/RANKL/OPGduring orthodontic tooth movement[J]. Orthod Craniofac Res, 2009, 12(2):113-119.

[22] Knop LA, Shintcovsk RL, Retamoso LB, et al. Non-steroidal and steroidal anti-inflammatory use in the context of orthodontic movement[J]. Eur J Orthod, 2012, 34(5):531-535.